The 5 strains that comprise this NOD congenic set (Stock No.'s 007930, 007931, 007932, 007933, 007934) contain the Idd 3 region from non-NOD strains. All of these strains are viable and fertile. Stocks containing the Idd 3 region from SWR/J, A/J, or CAST/EiJ are diabetes susceptible, while the congenic stocks containing the Idd 3 region derived from C57BL/6 or CZECHI/EiJ are diabetes resistant and produce more IL2 mRNA than the diabetes susceptible strains. These strains have been used to positionally clone Idd3 on Chr. 3 and continue to be valued to study the biological effects of variation of genes within the Idd3 region and for studying long-range chromosome remodeling.
Linda Wicker, University of Cambridge, UK
This NOD congenic strain, commonly referred to as NOD.SWR-Idd3, is carrying Chr 3 alleles, SNPs rs13476974 (11.10Mb) through rs13477217 (78.79Mb), derived from strain SWR/J including the insulin dependent diabetes susceptibility 3 loci (Idd3). Diabetes onset and incidence among mice of this strain reported reported to be similar to NOD/MrkTac. Thymocytes and splenocytes of this T1D susceptible stock produce 2 fold less Il2 mRNA than T1D protected strains (Stock No's. 007933 and 007934). This stock has been useful to positionally clone Idd3 and to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 gene and continue to be valued for studying long-range chromosome remodeling.
The SWR/J (Stock No. 000689) congenic segment is 67.69Mb in length and is defined within SNPs rs13476974 (11.10Mb) and rs13477217 (78.79Mb) that includes the Idd3 locus on Chr 3. Idd3 contains major candidate gene IL2 as well as IL21, Tenr, Cetn4 and Fgf2. The SWR/J congenic segment was introgressed onto the NOD/MrkTac background. This strain was produced by mating mice from NOD/MrkTac to SWR/J followed by 5 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the SWR/J derived segment denoted by makers D3Mit329 through D3Mit274 were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 36, identifies the congenic interval between SNPs rs13476974 (11.10Mb) and rs13477217 (78.79Mb) with no genetic contamination of other chromosomes. In 2007, the T1DR received this strain at generation N6F25.
|Gene Symbol and Name||Idd3, insulin dependent diabetes susceptibility 3|
|Strain of Origin||SWR/J|
|Molecular Note||This allele confers susceptibility to type 1 diabetes compared to C57BL/6J and CZECHI/EiJ. Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac.|