These Gli2flox mutant mice harbor loxP sites flanking exons 7 and 8 of the GLI-Kruppel family member GLI2 (Gli2) gene and may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs.
Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center
Mice homozygous for this Gli2flox conditional allele are viable and fertile, with loxP sites flanking exons 7 and 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have these exons deleted in the cre-expressing tissue(s). This results in a frameshift mutation following splicing of mRNA from exon 6 to 9 and is reported to confer the null phenotype. These Gli2flox mutant mice may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs.
A targeting vector was designed to place an Frt-flanked neo cassette followed by a loxP site upstream of exon 7, as well as a loxP site (containing an Asp718 site) downstream of exon 8 of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss Webster outbred mice. The resulting Gli2flox-neo heterozygotes were then bred to Swiss Webster mice constitutively expressing Flp recombinase (see Stock No. 003800) to remove the Frt-flanked neo cassette, generating Gli2flox offspring (with a single remaining Frt site just upstream of the loxP-flanked exons 7 and 8). These Gli2flox mutant mice were bred to Swiss Webster mice for many generations prior to arrival at The Jackson Laboratory. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, Gli2flox mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
|Allele Name||targeted mutation 6, Alexandra L Joyner|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Gli2, GLI-Kruppel family member GLI2|
|Gene Synonym(s)||AW546128; CJS; HPE9; PHS2; THP1; THP2; expressed sequence AW546128|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Two loxP sites were inserted flanking exons 7 and 8, which are upstream of the exons encoding the zinc fingers. A neo cassette flanked with Frt sites was also inserted upstream of exon 7 and then removed with Flp DNA recombinase.|
|Mutations Made By|| |
Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center
|Please inquire about possible genotypes.|
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