Mice homozygous for this Gli2lzki allele harbor a β-galactosidase "knock-in" (lzki) mutation that also abolishes endogenous GLI-Kruppel family member GLI2 (Gli2) gene function, and may be useful for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs.
Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Gli2 | GLI-Kruppel family member GLI2 |
Mice homozygous for this Gli2lzki allele harbor a β-galactosidase "knock-in" (lzki) allele that also abolishes endogenous gene function. As such, homozygous mice die at birth with many defects including absence of Sonic Hedgehog-expressing floor plate cells, reduction of Nkx2.2-expressing V3 interneurons in the spinal cord, and defects in midbrain, cerebellum and lung development. Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern identical to the wild-type gene. Heterozygotes are viable and fertile. These Gli2lzki mice may be useful for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs.
A targeting vector was designed with a nuclear localized β-galactosidase cDNA (with 1.7 kb of 3' UTR and three tandem SV40 poly A sequence repeats downstream) and followed by a reverse-oriented loxP-flanked neo cassette. This construct was used to replace a portion of the first coding exon (exon 2) of the targeted gene (including the ATG start codon) via electroporation into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss Webster outbred mice. The resulting Gli2nlzki heterozygotes were then bred to cre expressing mice (on a Swiss Webster genetic background) to remove the neo cassette, generating Gli2lzki offspring (with a single loxP site remaining after the SV40 polyA). These Gli2lzki mutant mice were bred to Swiss Webster mice for many generations prior to arrival at The Jackson Laboratory. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, Gli2lzki mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | LacZ expression is observed in a pattern identical to the wild-type targeted gene. |
Allele Name | targeted mutation 2.1, Alexandra L Joyner |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Gli2lacZ; Gli2lz; Gli2lzki |
Gene Symbol and Name | Gli2, GLI-Kruppel family member GLI2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | LacZ expression is observed in a pattern identical to the wild-type targeted gene. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 1 |
Molecular Note | A lacZ gene and a loxP-flanked neomycin selection cassette replaced 56 bp of exon 2, including the initiation codon. The neomycin selection cassette was removed by Cre mediated recombination in mice by crossing Gli2tm2Alj mice with NLS-cre expressing mice, and selecting for offspring that no longer had the neomycin cassette. |
Mutations Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings. Homozygotes die at birth.
When using the STOCK Gli2tm2.1Alj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007922 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Gli2<tm2.1Alj> |
Frozen Mouse Embryo | STOCK Gli2<tm2.1Alj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gli2<tm2.1Alj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gli2<tm2.1Alj>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gli2<tm2.1Alj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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