These En1flox mice harbor loxP-flanked homeodomain portion of exon 2 of the engrailed 1 (En1 locus. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). As such, these mice may be useful in generating conditional mutations for studying engrailed protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum, limbs, somites, and skin.
Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | En1 | engrailed 1 |
Mice homozygous for the En1flox conditional allele are viable and fertile, with loxP sites flanking the coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the engrailed homeodomain) deleted in the cre-expressing tissue(s). These En1flox mice may be useful in generating conditional mutations for studying engrailed protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum, limbs, somites, and skin.
Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007916, Stock No. 007917, Stock No. 007924, and Stock No. 007925).
A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase (see Stock No. 003800) to remove the Frt-flanked neo cassette, generating En1flox offspring. These En1flox mutant mice were bred together or to Swiss Webster mice for many generations prior to arrival at The Jackson Laboratory. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, En1flox mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 8.1, Alexandra L Joyner |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | En1flox |
Gene Symbol and Name | En1, engrailed 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 1 |
Molecular Note | A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring. |
Mutations Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center |
When maintaining a live colony, homozygous mice may be bred.
When using the STOCK En1tm8.1Alj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007918 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for En1<tm8.1Alj> |
Frozen Mouse Embryo | STOCK En1<tm8.1Alj>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK En1<tm8.1Alj>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK En1<tm8.1Alj>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK En1<tm8.1Alj>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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