The Cre-inducible expression of DTR in these iDTR knock-in mice render cells susceptible to ablation following Diphtheria toxin administration.
Ari Waisman, Johannes Gutenberg University of Mainz
Genetic Background | Generation |
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N?+pN2F15
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian diphtheria toxin receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. Of note, it has been the experience of The Jackson Laboratory that mice homozygous for the DTR, in the absence of Cre, are undersized at 3 weeks of age. We therefore recommend weaning these mice at ~4 weeks of age.
When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.
When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 011062), this mutant mouse strain may be useful in studies of ovarian development.
When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. 011069), this mutant mouse strain may be useful in studies of metabolic dysfunction.
Of note, iDTR mice are also available on a BALB/cBy congenic background (as Stock No. 008040) and a NOD congenic background (as Stock No. 016603).
Of note, The Jackson Laboratory Repository also distributes RC::L-DTA mice (Stock No. 026944), which have Cre recombinase-dependent inverted tox176 attenuated diphtheria toxin subunit alpha gene (DTA*G128D), as well as widespread eGFP fluorescence in the absence of Cre recombinase.
A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in CB20 blastocysts. Chimeric mice were bred to C57BL/6 to generate heterozygous "iDTR" mice. The donating investigator reported that these iDTR mice were maintained as homozygotes on the C57BL/6 genetic background (see SNP note below) prior to arrival at The Jackson Laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | DTR, Simian Diphtheria Toxin Receptor, |
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Expressed Gene | HBEGF, heparin binding EGF like growth factor, chimpanzee |
Site of Expression |
Allele Name | targeted mutation 1, Ari Waisman |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence) |
Allele Synonym(s) | Gt(ROSA)26Sortm1(Dtr)ThBu; iDTR; R26iDTR; ROSA26-DTR; Rosa26iDTR; Rosa26-LSL-DTR; RosaiDTR; Rosa-iDTR |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | DTR, Simian Diphtheria Toxin Receptor, |
Expressed Gene | HBEGF, heparin binding EGF like growth factor, chimpanzee |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 6 |
Molecular Note | A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an frt-flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. |
Mutations Made By | Ari Waisman, Johannes Gutenberg University of Mainz |
When maintaining a live colony, homozygous mice may be bred.
Homozygotes are typically undersized at 3 weeks of age. We therefore recommend weaning these mice at ~4 weeks of age.
When using the ROSA26iDTR mouse strain in a publication, please cite the originating article(s) and include JAX stock #007900 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(HBEGF)Awai> |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(HBEGF)Awai>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(HBEGF)Awai>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(HBEGF)Awai>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Gt(ROSA)26Sor<tm1(HBEGF)Awai>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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