B6;129P2-H2-M10.2^tm2Mom/MomJ

Stock number: 007878
Research areas: Neurobiology Research, Research Tools

Description

This strain provides a tool for the characterization of vomeronasal sensory neuron development and function.

Detailed description

This allele has green fluorescent protein fused to the C-terminus of H2-M10.2, permitting visualization of its expression pattern. 95% of the subset of vomeronasal sensory neurons that express H2-Mv genes express H2-M10.2. Expression of H2-M10.2 is found in both dendrites and axons of vomeronasal sensory neurons. Immunohistochemistry shows expression at the vomeronasal organ dendrite tip, but not at synaptic terminals in the accessory olfactory bulb. At five weeks of age this protein is abundantly present at microvilli in the vomeronasal organ and can also be found in the glomeruli of the accessory olfactory bulb.

Development

This mutation was generated in 129P2/OlaHsd derived E14 ES cells and the ACNf neomycin cassette was self-excised by cre recombination in the germline of the male chimera. Outcrossing to C57BL/6J preceded intercrossing to homozygosity. In 2007 sperm were cryopreserved from homozygous males on this mixed B6;129P2 background

Allele

Symbol: H2-M10.2^tm2Mom
Name: targeted mutation 2, Peter Mombaerts
MGI accession ID: MGI:3722350
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: H2-M10.2
Marker name: histocompatibility 2, M region locus 10.2
Chromosome: 17
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Vomeronasal sensory neurons
Molecular note: A targeting vector was constructed with an M10.2-GFP-ACNF (ACE-cre/neo-loxP) cassette inserted before the stop codon of the BAC M10.2. The ACNF, a self excising neo cassete, was removed during transmission through the male germline, leaving a single loxP site.