C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J

Stock number: 007857
Research areas: Cell Biology Research, Research Tools

Description

These transgenic mice express Yellow Fluorescent Protein (YFP) under the control of the rat, enolase 2, gamma, Eno2, promoter. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, gene targeting signal fused to the N-terminus. This mutant mouse strain may be useful in studies of mitochondrial transport and neuronal metabolism.

Detailed description

These transgenic mice express Yellow Fluorescent Protein (YFP) under the control of the neuron specific rat enolase 2, gamma, Eno2, promoter. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations. The donating investigator reports that fluorescence is detected in subcortical structures including the thalamus, basal ganglia, and caudate/putamen; interneurons of the cortex and hippocampus; a subset of retinal ganglion cells; cerebellum mossy fiber rosette terminals of the granule cell layer and spinal cord preganglionic autonomic neurons. It is not known if homozygotes are viable. This mutant mouse strain may be useful in studies of mitochondrial transport and neuronal metabolism.

Development

A transgenic construct containing the entire open reading frame of the Yellow Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the mouse cytochrome c oxidase subunit 8 (ubiquitous) gene under the direction of regulatory elements derived from the rat enolase 2, gamma gene, Eno2, was injected into fertilized C57BL/6J donor mouse eggs. Founder line 3R12, MitoY, corresponding to the published line Y, was subsequently established.

Allele

Symbol: Tg(Eno2-YFP/Cox8a)YRwb
Name: transgene insertion Y, Robert Burgess
MGI accession ID: MGI:3722776
Generation method: Transgenic
Attributes: Reporter
Synonyms: 3R12; MitoY
Marker symbol: Tg(Eno2-YFP/Cox8a)YRwb
Marker name: transgene insertion Y, Robert Burgess
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: YFP is specifically localized to the mitochondria in many neuronal populations. Fluorescence is reported in subcortical structures including the thalamus, basal ganglia, and caudate/putamen; interneurons of the cortex and hippocampus; a subset of retinal ganglion cells; cerebellum mossy fiber rosette terminals of the granule cell layer and spinal cord preganglionic autonomic neurons.
Molecular note: A transgenic construct containing the entire open reading frame of the Yellow Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the mouse cytochrome c oxidase subunit 8 (ubiquitous) gene under the direction of regulatory elements derived from the rat enolase 2, gamma gene, Eno2, was injected into fertilized C57BL/6J donor mouse eggs. Founder line Y was established. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations.