These transgenic mice express Yellow Fluorescent Protein (YFP) under the control of the rat, enolase 2, gamma, Eno2, promoter. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, gene targeting signal fused to the N-terminus. This mutant mouse strain may be useful in studies of mitochondrial transport and neuronal metabolism.
Robert Burgess, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice express Yellow Fluorescent Protein (YFP) under the control of the neuron specific rat enolase 2, gamma, Eno2, promoter. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations. The donating investigator reports that fluorescence is detected in subcortical structures including the thalamus, basal ganglia, and caudate/putamen; interneurons of the cortex and hippocampus; a subset of retinal ganglion cells; cerebellum mossy fiber rosette terminals of the granule cell layer and spinal cord preganglionic autonomic neurons. It is not known if homozygotes are viable. This mutant mouse strain may be useful in studies of mitochondrial transport and neuronal metabolism.
A transgenic construct containing the entire open reading frame of the Yellow Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the mouse cytochrome c oxidase subunit 8 (ubiquitous) gene under the direction of regulatory elements derived from the rat enolase 2, gamma gene, Eno2, was injected into fertilized C57BL/6J donor mouse eggs. Founder line 3R12, MitoY, corresponding to the published line Y, was subsequently established.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | YFP is specifically localized to the mitochondria in many neuronal populations. Fluorescence is reported in subcortical structures including the thalamus, basal ganglia, and caudate/putamen; interneurons of the cortex and hippocampus; a subset of retinal ganglion cells; cerebellum mossy fiber rosette terminals of the granule cell layer and spinal cord preganglionic autonomic neurons. |
Allele Name | transgene insertion Y, Robert Burgess |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | 3R12; MitoY |
Gene Symbol and Name | Tg(Eno2-YFP/Cox8a)YRwb, transgene insertion Y, Robert Burgess |
Gene Synonym(s) | |
Promoter | Eno2, enolase 2, gamma, neuronal, rat |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | YFP is specifically localized to the mitochondria in many neuronal populations. Fluorescence is reported in subcortical structures including the thalamus, basal ganglia, and caudate/putamen; interneurons of the cortex and hippocampus; a subset of retinal ganglion cells; cerebellum mossy fiber rosette terminals of the granule cell layer and spinal cord preganglionic autonomic neurons. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A transgenic construct containing the entire open reading frame of the Yellow Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the mouse cytochrome c oxidase subunit 8 (ubiquitous) gene under the direction of regulatory elements derived from the rat enolase 2, gamma gene, Eno2, was injected into fertilized C57BL/6J donor mouse eggs. Founder line Y was established. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations. |
When maintaining a live colony, these mice can be bred as hemizygotes. It is not known if homozygotes are viable.
When using the C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007857 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non-carrier for Tg(Eno2-YFP/Cox8a)YRwb |
Frozen Mouse Embryo | C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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