Overview

This strain has been discontinued. The Myf5^tm3(cre)Sor targeted mutation has been cryopreserved under Stock No. 007893 B6.129S4-Myf5^tm3(cre)Sor/J

This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. This mutant mouse strain represents a model that may be useful in studies of skeletal development.

Donating Investigator

Dr. Philippe Soriano, Mount Sinai School of Medicine

Genetic overview

Genetic Background	Generation

Myf5^tm3(cre)Sor

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Myf5	myogenic factor 5

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Research Applications

Developmental Biology Research
Research Tools

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Details

Detailed Description

This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development.

Development

A targeting vector containing nuclear localized cre coding sequence, loxP site flanked neomycin resistance cassette and a diphtheria toxin gene was inserted 90 bp upstream of the initiation codon to disrupt the targeted gene. The endogenous Myf5 promoter drives expression of the Cre recombinase. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Cre recombinase expressing strain to remove the neomycin selection cassette and then backcrossed to C57BL/6J mice for one generation.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	skeletal muscle, dermis, and several ectopic locations

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Tallquist MD; Weismann KE; Hellstrom M; Soriano P. 2000. Early myotome specification regulates PDGFA expression and axial skeleton development Development 127(23):5059-70PubMed: 11060232 MGI: J:65301

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Genetics

Myf5^tm3(cre)Sor

Allele Symbol: Myf5^tm3(cre)Sor

Allele Name	targeted mutation 3, Philippe Soriano
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Mif5-Cre; Myf5^Cre; Myf5^Cre-So; Myf5^CreSOR; myf5-CreTG
Gene Symbol and Name	Myf5, myogenic factor 5
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	skeletal muscle, dermis, and several ectopic locations
Strain of Origin	129S4/SvJaeSor
Chromosome	10
Molecular Note	A cre cDNA was inserted between a SacI site and a KpnI site in the 5'UTR of the Myf5 gene. An adjacent loxP-flanked neomycin cassette was removed by Cre- mediated recombination.
Mutations Made By	Dr. Philippe Soriano, Mount Sinai School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Perinatal Lethality
  - Homozygous
- Skeletal Defects
Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Developmental Biology Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Myf5^tm3(cre)Sor/Myf5^tm3(cre)Sor
involves: 129S4/SvJae * C57BL/6J

skeleton phenotype

thoracic vertebral fusion
- fusions are seen in most animals, but are less severe than in Myf5^tm1Jae homozygotes

cervical vertebral fusion
- fusions are seen in most animals, but are less severe than in Myf5^tm1Jae homozygotes

abnormal rib development
- animals show more rib formation than Myf5^tm1Jae homozygotes, but ribs frequently do not extend to the vertebrae

References

Tallquist MD; Weismann KE; Hellstrom M; Soriano P. 2000. Early myotome specification regulates PDGFA expression and axial skeleton development Development 127(23):5059-70PubMed: 11060232 MGI: J:65301

Additional - Myf5^tm3(cre)Sor related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Myf5
- Standard PCR:Myf5alternate1
- Standard PCR:Myf5
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have a perinatal lethal phenotype.
- Additional Breeding and Husbandry Support

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Myf5tm3(cre)Sor

Allele Symbol: Myf5tm3(cre)Sor

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Myf5tm3(cre)Sor/Myf5tm3(cre)Sorinvolves: 129S4/SvJae * C57BL/6J

skeleton phenotype

References

Additional - Myf5tm3(cre)Sor related

Genotyping Protocols

Breeding Considerations

Terms Of Use

Licensing Information

Myf5^tm3(cre)Sor

Allele Symbol: Myf5^tm3(cre)Sor

Genotype: Myf5^tm3(cre)Sor/Myf5^tm3(cre)Sor
involves: 129S4/SvJae * C57BL/6J

Additional - Myf5^tm3(cre)Sor related