B6;129S4-Myf5^tm3(cre)Sor/J

Stock number: 007845
Research areas: Developmental Biology Research, Research Tools

Description

This strain has been discontinued. The Myf5^tm3(cre)Sor targeted mutation has been cryopreserved under Stock No. 007893 B6.129S4-Myf5^tm3(cre)Sor/J

This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. This mutant mouse strain represents a model that may be useful in studies of skeletal development.

Detailed description

This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development.

Development

A targeting vector containing nuclear localized cre coding sequence, loxP site flanked neomycin resistance cassette and a diphtheria toxin gene was inserted 90 bp upstream of the initiation codon to disrupt the targeted gene. The endogenous Myf5 promoter drives expression of the Cre recombinase. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Cre recombinase expressing strain to remove the neomycin selection cassette and then backcrossed to C57BL/6J mice for one generation.

Allele

Symbol: Myf5^tm3(cre)Sor
Name: targeted mutation 3, Philippe Soriano
MGI accession ID: MGI:2135565
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Myf5
Marker name: myogenic factor 5
Chromosome: 10
Strain of origin: 129S4/SvJaeSor
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: skeletal muscle, dermis, and several ectopic locations
Molecular note: A cre cDNA was inserted between a SacI site and a KpnI site in the 5'UTR of the Myf5 gene. An adjacent loxP-flanked neomycin cassette was removed by Cre- mediated recombination.