These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Efnb2 | ephrin B2 |
These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development.
A targeting vector containing the histone 2B and Green Fluorescent Protein, H2BGFP, fusion gene was used to disrupt exon 1 and the start codon of the Efnb2 gene. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Fluorescence patterns have not been characterized. |
Allele Name | targeted mutation 2, Philippe Soriano |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | B2GFP; Efnb2GFP; Efnb2-H2BGFP; ephrin-B2GFP |
Gene Symbol and Name | Efnb2, ephrin B2 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Fluorescence patterns have not been characterized. |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 8 |
Molecular Note | The H2B-GFP cDNA was inserted into exon 1 replacing a fragment of DNA including the start codon. RT-PCR analysis confirmed the absence of RNA expression in homozygous embryos. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype and die at E10.5 due to cardiovascular defects.
When using the B6;129S4-Efnb2tm2Sor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007843 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Efnb2<tm2Sor> |
Frozen Mouse Embryo | B6;129S4-Efnb2<tm2Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Efnb2<tm2Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Efnb2<tm2Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Efnb2<tm2Sor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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