B6;129S4-Efnb2^tm2Sor/J

Stock number: 007843
Research areas: Developmental Biology Research, Research Tools

Description

These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development.

Detailed description

These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development.

Development

A targeting vector containing the histone 2B and Green Fluorescent Protein, H2BGFP, fusion gene was used to disrupt exon 1 and the start codon of the Efnb2 gene. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice.

Allele

Symbol: Efnb2^tm2Sor
Name: targeted mutation 2, Philippe Soriano
MGI accession ID: MGI:3526818
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Efnb2
Marker name: ephrin B2
Chromosome: 8
Strain of origin: 129S4/SvJaeSor
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Fluorescence patterns have not been characterized.
Molecular note: The H2B-GFP cDNA was inserted into exon 1 replacing a fragment of DNA including the start codon. RT-PCR analysis confirmed the absence of RNA expression in homozygous embryos.