STOCK H2az2^{Tg(Wnt1-cre)11Rth} Tg(Wnt1-GAL4)11Rth/MileJ

Stock number: 007807
Product name: Wnt-1/GAL4/cre-11
Research areas: Neurobiology Research, Research Tools

Description

In 2011, Stock No. 007807 at The Jackson Laboratory Repository was confirmed to harbor the expected Wnt1-Cre transgene, as well as the originally co-injected Wnt1-GAL4 transgene. Because it is redundant to Stock No. 003829, Stock No. 007807 has been terminated.

Allele

Symbol: Tg(Wnt1-GAL4)11Rth
Name: transgene insertion 11, David H Rowitch
MGI accession ID: MGI:3524966
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: WEXP-GAL4; Wnt-1/GAL4
Marker symbol: Tg(Wnt1-GAL4)11Rth
Marker name: transgene insertion 11, David H Rowitch
Marker synonyms: WEXP-GAL4; Wnt-1/GAL4
Chromosome: UN
Strain of origin: (C57BL/6J x CBA/J)F1
Expressed genes: GAL4,transcriptional activator GAL4,yeast
Site of expression: embryonic neural tube, midbrain, dorsal and ventral midlines of the midbrain and caudal diencephalon, the mid-hindbrain junction and dorsal spinal cord
Molecular note: The transgene contains the yeast GAL4 gene directed by Wnt1 promoter/enhancer sequences. Expression of the transgene matches that of the endogenous Wnt1.
General note: Six lines were generated.

Homozygous transgenic mice that are also homozygous for Tg(Wnt1-cre)11Rth are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Both Cre recombinase and the GAL4 transcriptional activator are expressed under the direction of Wnt1 regulatory sequences. Regulated expression initially occurs in the midbrain. After neural tube closure, expression occurs in the dorsal and ventral midlines of the midbrain and caudal diencephalon, the midbrain-hindbrain junction, and in the dorsal spinal cord.

Allele

Symbol: H2az2^{Tg(Wnt1-cre)11Rth}
Name: transgene insertion 11, David H Rowitch
MGI accession ID: MGI:2386570
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: Tg(Wnt1-cre)11Rth; Wnt1::Cre; Wnt1^Cre; Wnt1cre; Wnt1-Cre
Marker symbol: H2az2
Marker name: H2A.Z histone variant 2
Marker synonyms: C530002L11Rik; H2A.Z2; H2A.Z-2; H2afv; H2AV
Chromosome: 11
Strain of origin: (C57BL/6J x CBA/J)F1/J
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: embryonic neural tube, midbrain, dorsal and ventral midlines of the midbrain and caudal diencephalon, the mid-hindbrain junction and dorsal spinal cord
Molecular note: The Wnt1 promoter preceded the cre recombinase coding sequence which was followed downstream by the Wnt1 enhancer. The Wnt1 regulatory sequences initially direct expression in the midbrain. After neural tube closure, expression occurs in the dorsal and ventral midlines of the midbrain and caudal diencephalon, the midbrain-hindbrain junction and in the dorsal spinal cord. Line 11 inserted into the gene at 6425500-6456783 (Build GRCm38/mm10) resulting in a 31,283 bp deletion. Founder line x has a copy number of greater than 5.
General note: Homozygous transgenic mice that are also homozygous for Tg(Wnt1-GAL4)11Rth are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.