These MIP-Luc-VU mice have luciferase (luc) expression under control of the mouse insulin I promoter (MIP or Ins1) and may be useful as a source of luciferase-expressing β cells for pancreatic islet cultures and transplantation, as well as non-invasive, in vivo monitoring of bioluminescent β cells for studying glucose homeostasis, β cell growth and regeneration, and diabetes.
Alvin C Powers, Vanderbilt University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for this MIP-luc transgene are viable and fertile, with luciferase (luc) expression under control of the mouse insulin I promoter (MIP or Ins1). After injection of the luciferin substrate, luc expression can be visualized using bioluminescence imaging (BLI). Mice from founder line VU (MIP-Luc-VU) exhibit strong and consistent luc bioluminescence emanating exclusively from β cells of the pancreatic islet, and luc-expressing beta cells can be visualized through the skin. MIP-Luc-VU pancreatic islets have normal islet architecture and insulin secretion both in vivo and in vitro, with luciferase intensity reporting the number of islets. In transplantation settings and models of increased/decreased beta cell mass, bioluminescence is proportional to beta cell mass (incorporating some aspects of islet function, with signal up-regulation in hyperglycemic states). These MIP-Luc-VU mice may be useful as a source of luciferase-expressing β cells for pancreatic islet cultures and transplantation, as well as non-invasive, in vivo monitoring of bioluminescent β cells for studying glucose homeostasis, β cell growth and regeneration, and diabetes.
The MIP-luc transgenic construct was designed with the 9.2 kb mouse insulin I promoter (MIP or Ins1) and a luciferase (luc) optical reporter cDNA gene (with a β-globin region and 3' non-transgenic region flanking the luc fragment on each side to stabilize the transgene). This transgene was injected into FVB/N embryos. Mice from founder line "VU" (specifically 43-8 (line 5)) exhibited the highest and most consistent luciferase expression over the pancreatic region and were found to have approximately three copies. These MIP-Luc-VU mice were maintained on the FVB/N genetic background by breeding with wild-type siblings for many generations prior to arrival at The Jackson Laboratory.
Expressed Gene | luc, luciferase, firefly |
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Site of Expression | Induced luciferase (luc) is expressed exclusively from β cells of the pancreatic islet, visible through the skin. |
Allele Name | transgene insertion VU, Alvin Powers |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | MIP-Luc-VU |
Gene Symbol and Name | Tg(Ins1-luc)VUPwrs, transgene insertion VU, Alvin Powers |
Gene Synonym(s) | |
Promoter | Ins1, insulin I, mouse, laboratory |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | Induced luciferase (luc) is expressed exclusively from β cells of the pancreatic islet, visible through the skin. |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | The MIP-luc transgenic construct was designed with the 9.2 kb mouse insulin I promoter (MIP or Ins1) and a luciferase (luc) optical reporter cDNA gene (with a rabbit beta-globin intronic and polyA sequences and 3' non-transgenic region flanking the luc fragment on each side to stabilize the transgene). This transgene was injected into FVB/N embryos. Mice from founder line "VU" (specifically 43-8 (line 5)) exhibited the highest and most consistent luciferase expression over the pancreatic region and were found to have approximately three copies. |
Mutations Made By | Alvin Powers, Vanderbilt University |
When maintaining a live colony, hemizygous mice are bred with wildtype siblings or with FVB/NJ (Stock No. 001800).
When using the FVB/N-Tg(Ins1-luc)VUPwrs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007800 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ins1-luc)VUPwrs |
Frozen Mouse Embryo | FVB/N-Tg(Ins1-luc)VUPwrs/J Embryos | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Ins1-luc)VUPwrs/J Embryos | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Ins1-luc)VUPwrs/J Embryos | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(Ins1-luc)VUPwrs/J Embryos | $3373.50 |
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