These transgenic mice express Cre recombinase under the direction of an activation-induced cytidine deaminase promoter. Recombinase activity results in the deletion of loxP-flanked targets in antigen-experienced B Cells; crosses of this strain with Cre-reporter mice (e.g. EYFP signal) can enable labeling of this subset of B cells. This mouse line can also be used to conditionally inactivate genes in germinal center B cells through Cre/loxP-mediated recombination. Splenic B cells express Cre recombinase after in vitro stimulation with lipopolysaccharide (LPS) and interleukin 4 (IL-4). B cells obtained from mice homozygous for this Cre insertion behave like homozygous targeted (knockout) mice and are unable to undergo class switch recombination (CSR) in vitro.
Michel C Nussenzweig, The Rockefeller University
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the direction of an activation-induced cytidine deaminase promoter. Recombinase activity results in the deletion of loxP-flanked targets in antigen-experienced B Cells; crosses of this strain with Cre-reporter mice (e.g. EYFP signal) can enable labeling of this subset of B cells. This mouse line can also be used to conditionally inactivate genes in germinal center B cells through Cre/loxP-mediated recombination. Splenic B cells express Cre recombinase after in vitro stimulation with lipopolysaccharide (LPS) and interleukin 4 (IL-4). B cells obtained from mice homozygous for this Cre insertion behave like homozygous targeted (knockout) mice and are unable to undergo class switch recombination (CSR) in vitro.
A targeting vector containing Cre, an FRT-flanked neomycin resistance cassette, and a DTA (diptheria toxin) gene was used to replace exon 1 of the gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The FRT-flanked neomycin cassette was excised from resultant mice through crosses with a Flp strain. A stable mouse line carrying Cre recombinase was backcrossed to C57BL/6 for 11 generations by the donating lab.
|Expressed Gene||cre, cre recombinase, bacteriophage P1|
|Site of Expression|
|Allele Name||targeted mutation 1, Michel C Nussenzweig|
|Allele Type||Targeted (Recombinase-expressing)|
|Allele Synonym(s)||AID Cre; AID-; AIDCre|
|Gene Symbol and Name||Aicda, activation-induced cytidine deaminase|
|Gene Synonym(s)||AID; ARP2; Aid; Aid; CDA2; HEL-S-284; HIGM2; activation induced deaminase; aid|
|Expressed Gene||cre, cre recombinase, bacteriophage P1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector containing Cre, an FRT-flanked neomycin resistance cassette, and a DTA (diphtheria toxin) gene was used to replace exon 1 of the gene.|
|Mutations Made By|| |
Michel Nussenzweig, The Rockefeller University
When maintained as a live colony, homozygotes may be bred.
When using the AID Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #007770 in your Materials and Methods section.
|Heterozygous for Aicda<tm1(cre)Mnz>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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