These transgenic mice express Cre recombinase under the direction of an activation-induced cytidine deaminase promoter. Recombinase activity results in the deletion of loxP-flanked targets in antigen-experienced B Cells; crosses of this strain with Cre-reporter mice (e.g. EYFP signal) can enable labeling of this subset of B cells. This mouse line can also be used to conditionally inactivate genes in germinal center B cells through Cre/loxP-mediated recombination. Splenic B cells express Cre recombinase after in vitro stimulation with lipopolysaccharide (LPS) and interleukin 4 (IL-4). B cells obtained from mice homozygous for this Cre insertion behave like homozygous targeted (knockout) mice and are unable to undergo class switch recombination (CSR) in vitro.
Michel C Nussenzweig, The Rockefeller University
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the direction of an activation-induced cytidine deaminase promoter. Recombinase activity results in the deletion of loxP-flanked targets in antigen-experienced B Cells; crosses of this strain with Cre-reporter mice (e.g. EYFP signal) can enable labeling of this subset of B cells. This mouse line can also be used to conditionally inactivate genes in germinal center B cells through Cre/loxP-mediated recombination. Splenic B cells express Cre recombinase after in vitro stimulation with lipopolysaccharide (LPS) and interleukin 4 (IL-4). B cells obtained from mice homozygous for this Cre insertion behave like homozygous targeted (knockout) mice and are unable to undergo class switch recombination (CSR) in vitro.
A targeting vector containing Cre, an FRT-flanked neomycin resistance cassette, and a DTA (diptheria toxin) gene was used to replace exon 1 of the gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The FRT-flanked neomycin cassette was excised from resultant mice through crosses with a Flp strain. A stable mouse line carrying Cre recombinase was backcrossed to C57BL/6 for 11 generations by the donating lab.
|Expressed Gene||cre, cre recombinase, bacteriophage P1|
|Site of Expression|
|Allele Name||targeted mutation 1, Michel C Nussenzweig|
|Allele Type||Targeted (Recombinase-expressing)|
|Allele Synonym(s)||AID Cre; AID-; AIDCre|
|Gene Symbol and Name||Aicda, activation-induced cytidine deaminase|
|Gene Synonym(s)||AID; ARP2; Aid; Aid; CDA2; HEL-S-284; HIGM2; activation induced deaminase; aid|
|Expressed Gene||cre, cre recombinase, bacteriophage P1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector containing Cre, an FRT-flanked neomycin resistance cassette, and a DTA (diphtheria toxin) gene was used to replace exon 1 of the gene.|
|Mutations Made By|| |
Michel Nussenzweig, The Rockefeller University
When maintained as a live colony, homozygotes may be bred.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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