This congenic NOD strain contains a transgene encoding murine Cd80 controlled by IgM enhancer and promoter elements and is commonly referred to as NOD.B7-1B Tg. The donating investigator reports that this diabetes resistant strain expresses high levels of the transgene on B cells, but not on T-cells. Circulating, speenic, and bone marrow B cells are significantly reduced. This strain may be used to study autoreactive T cell activation, B cell deletion and the role of B7 co stimulatory molecules in autoimmunity, specifically Type 1 Diabetes.
Dr. Jeffrey A. Bluestone, University of California, San Francisco
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities. High levels of the transgene are expressed on B cells, but not on T cells. At 30 weeks of age transgenic mice are diabetes resistant and insulitis was significantly reduced when compared with wildtype NOD controls. When compared to wildtype NOD controls, the circulating B cells in congenic NOD transgenic mice is 2-3 fold lower in 2 week old mice and 10 fold lower in 5-6 week old mice and persists throughout life. Significantly reduced percentage of B cells were found in the spleen and bone marrow. Analysis of bone marrow shows the more mature B cell subsets (B220+, IgM+) are affected. Elisa tests indicate reduced circulating levels of all Ig types, Ighm (formerly Igh-6), Igh-3 (IgG2b), and Ighg1 (IgG1) in the serum of transgenic mice. MHC class II expression of splenic B cells was significantly increased by more than 2 fold, indicating features of an activated B cell phenotype, in NOD-H2g7 transgenic mice when compared to NOD wildtype and NOD.-H2b transgenic mice.
In 2008, a spontaneous mutation to dystonia (Dst) was observed in this colony and may be segregating in cryopreserved stock.
This strain may be used to study autoreactive T cell activation and B cell deletion and the role of B7 costimulatory molecules in autoimmunity, specifically Type 1 Diabetes.
The Igh-6-CD80 transgenic construct, commonly called B7-1B Tg, encodes a murine Cd80 cDNA, containing the complete coding region that lacks the 2' and 5' UTR's, controlled by IgM enhancer and promoter elements. To enhance expression of the transgene, the construct additionally contains a mutated non-expressible segment of the human growth hormone gene. The transgenic construct was microinjected into FVB/N embryos. FVB/N transgenic mice from line B-B7-1 were backcrossed to NOD/MrkTac mice for more than 15 generations. In 2007, the T1DR received transgenic mice at generation N15+ and backcrossed to NOD/ShiLtJ (Stock No. 001976) once prior to sibling mating.
Expressed Gene | Cd80, CD80 antigen, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Gordon J Freeman |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | B.B7-1; B7-1 transgene; B7-1T Tg; T.B7-1 |
Gene Symbol and Name | Tg(Igh-6-Cd80)1Gjf, transgene insertion 1, Gordon J Freeman |
Gene Synonym(s) | |
Promoter | Ighm, immunoglobulin heavy constant mu, mouse, laboratory |
Expressed Gene | Cd80, CD80 antigen, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The transgene was constructed with the Cd80 cDNA containing the complete coding region (but lacking the 2' and 5' UTRs) under control of the Igh-6 enhancer and promoter elements. The construct also contained a mutated nonexpressible segment of the human growth hormone gene included to enhance transgene expression. |
Mutations Made By | Gordon Freeman, Harvard Medical School |
When using the NOD.FVB-Tg(Igh-6-Cd80)1Gjf/JbsJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #007769 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(Igh-6-Cd80)1Gjf/JbsJ |
Frozen Mouse Embryo | NOD.FVB-Tg(Igh-6-Cd80)1Gjf/JbsJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | NOD.FVB-Tg(Igh-6-Cd80)1Gjf/JbsJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | NOD.FVB-Tg(Igh-6-Cd80)1Gjf/JbsJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | NOD.FVB-Tg(Igh-6-Cd80)1Gjf/JbsJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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