The P2-IRES-tau-lacZ knockin allele is designed to express tau-lacZ fusion protein from the olfactory receptor 17 (Olfr17) promoter/enhancer regions. These B6-P2-IRES-tau-lacZ mice may be useful to visualize the axonal projections from specific subpopulations of olfactory neurons.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Olfr17 | olfactory receptor 17 |
The Olfr17tm1Mom knockin allele has a an internal ribosome entry site and the microtubule-binding protein tau / beta-galactosidase (tau-lacZ) fusion protein downstream of the olfactory receptor 17 gene (Olfr17). Olfactory sensory neurons that express the olfactory receptor 17 also co-express the tau-lacZ fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies. This reporter allele is combined with other olfactory receptor reporter alleles in Stock Number 027730.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of the Olfr17tm1Mom mice could vary from that originally described for Olfr17tm1Mom mice on a mixed B6;129P2 background. We may modify the strain description if necessary as published results become available.
The P2-KO-13 targeting vector was designed to insert an internal ribosome entry site (IRES), a tau-lacZ fusion protein, and a loxP-flanked tk-neo cassette (LTNL) all downstream of the endogenous coding sequence. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The loxP-flanked neo cassette was then removed by transient transfection of a plasmid expressing cre recombinase, prior to injection of these ES cells into blastocysts. The resulting P2-IRES-tau-lacZ mice were bred to C57BL/6 and intercrossed. Next, mutant mice were backcrossed more than ten generations to C57BL/6J prior to sending to The Jackson Laboratory Repository.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | lacZ is expressed in olfactory sensory neurons. |
Allele Name | targeted mutation 1, Peter Mombaerts |
---|---|
Allele Type | Targeted (Reporter) |
Allele Synonym(s) | OR-lacZ; P2*-LacZ; P2TL; P2-IRES-tau::lacZ; P2-IRES-taulacZ |
Gene Symbol and Name | Olfr17, olfactory receptor 17 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in olfactory sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | Insertion of an IRES-tau-lacZ-loxP-tk-neo-loxP expression cassette placed the inserted sequences under control of the endogenous regulatory sequences. Transient expression of Cre recombinase in C57BL/6 blastocysts excised the thymidine kinase and neomycin sequences. This allele expresses a bicistronic mRNA encoding the receptor and a bovine tau-beta-galactosidase fusion protein. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the B6.129P2-Olfr17tm1Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #007767 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Olfr17<tm1Mom> |
Frozen Mouse Embryo | B6.129P2-Olfr17<tm1Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Olfr17<tm1Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Olfr17<tm1Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Olfr17<tm1Mom>/MomJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.