These R26ΦC31o (R26phiC31o) mutant mice express a site-specific recombinase, ΦC31o (PhiC31o), that has been targeted to the endogenous Gt(ROSA)26Sor locus. The ΦC31o is codon-optimized for expression in mouse tissues. When R26ΦC31o mice are bred with mice containing AttB/AttP-flanked sequences, the resulting offspring will have the targeted sequence deleted and replaced with a single AttL site in widespread cells/tissues.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These R26ΦC31o (R26phiC31o) mutant mice express a site-specific recombinase, ΦC31o (PhiC31o), that has been targeted to the endogenous Gt(ROSA)26Sor locus. The ΦC31o is codon-optimized for expression in mouse tissues. ΦC31o mediates irreversible DNA recombination at specific nonidentical pairs of sequence sites, AttB and AttP; this results in deletion of the entire AttB/AttP-flanked sequences and replacement with a single AttL site. Recombination efficiency is similar to that of Cre recombinase. When R26ΦC31o mice are bred with mice containing AttB/AttP-flanked sequences, the resulting offspring will have the targeted sequence deleted in widespread cells/tissues.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting construct with the SA-PhiC310pA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus via electroporation of 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. Correctly targeted ES cells were identified and used to generate mutant mice. Homozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory (as Stock No. 007670). Upon arrival, some mice were backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least five generations to generate this congenic strain (Stock No. 007743).
Expressed Gene | phiC31, PhiC31 recombinase, |
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Site of Expression |
Allele Name | targeted mutation 3, Philippe Soriano |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | R26phiC31o |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | phiC31, PhiC31 recombinase, |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 6 |
Molecular Note | A targeting construct with the SA-PhiC31opA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus. Flp-mediated recombination removes the neo cassette. PhiC31o-mediated recombination in mice carrying containing a sequence of interest flanked by attB and attP sites results in tissue-specific deletion of the targeted sequence. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007743 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous of wildtype for Gt(ROSA)26Sor<tm3(phiC31*)Sor> |
Frozen Mouse Embryo | B6.129S4-Gt(ROSA)26Sor<tm3(phiC31*)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Gt(ROSA)26Sor<tm3(phiC31*)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Gt(ROSA)26Sor<tm3(phiC31*)Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Gt(ROSA)26Sor<tm3(phiC31*)Sor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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