B6.129S4-Gt(ROSA)26Sor^{tm3(phiC31*)Sor}/J

Stock number: 007743
Product name: R26ΦC31o
Research areas: Research Tools

Description

These R26ΦC31o (R26phiC31o) mutant mice express a site-specific recombinase, ΦC31o (PhiC31o), that has been targeted to the endogenous Gt(ROSA)26Sor locus. The ΦC31o is codon-optimized for expression in mouse tissues. When R26ΦC31o mice are bred with mice containing AttB/AttP-flanked sequences, the resulting offspring will have the targeted sequence deleted and replaced with a single AttL site in widespread cells/tissues.

Detailed description

These R26ΦC31o (R26phiC31o) mutant mice express a site-specific recombinase, ΦC31o (PhiC31o), that has been targeted to the endogenous Gt(ROSA)26Sor locus. The ΦC31o is codon-optimized for expression in mouse tissues. ΦC31o mediates irreversible DNA recombination at specific nonidentical pairs of sequence sites, AttB and AttP; this results in deletion of the entire AttB/AttP-flanked sequences and replacement with a single AttL site. Recombination efficiency is similar to that of Cre recombinase. When R26ΦC31o mice are bred with mice containing AttB/AttP-flanked sequences, the resulting offspring will have the targeted sequence deleted in widespread cells/tissues.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting construct with the SA-PhiC310pA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus via electroporation of 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. Correctly targeted ES cells were identified and used to generate mutant mice. Homozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory (as Stock No. 007670). Upon arrival, some mice were backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least five generations to generate this congenic strain (Stock No. 007743).

Allele

Symbol: Gt(ROSA)26Sor^{tm3(phiC31*)Sor}
Name: targeted mutation 3, Philippe Soriano
MGI accession ID: MGI:3766932
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129S4/SvJaeSor
Expressed genes: phiC31,PhiC31 recombinase,
Molecular note: A targeting construct with the SA-PhiC31opA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus. Flp-mediated recombination removes the neo cassette. PhiC31o-mediated recombination in mice carrying containing a sequence of interest flanked by attB and attP sites results in tissue-specific deletion of the targeted sequence.