These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.
For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 7 of the targeted gene, and another loxP site was inserted upstream of exon 7. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES). Homozygotes on a mixed B6;129P2 genetic background were sent to The Jackson Laboratory (as Stock No. 007605). Upon arrival, some mice were backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least 5 generations to generate this congenic strain (Stock No. 007685)(see note below).
NOTE: For the parental strain (Stock No. 007605), a 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed a single loci that typed as homozygous for 129/P strain type (Chromosome 12 approximately 9.8 cM) and three additional loci that were segregating for either C57BL/6J or 129/P strain type (Chromosome 1 approximately 52.0 cM, Chromosome 4 approximately 10.9 cM, and Chromosome 8 approximately 4.4 cM). These loci are to be monitored and attempted to be changed to C57BL/6J-like during the backcrossing protocol to generate the congenic strain (Stock No. 007685). Because the targeted mutation also resides on chromosome 12, that loci may remain as 129/P strain type even after backcrossing (August 2008).
|Allele Name||targeted mutation 1, Fred Van Leuven|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Psen1, presenilin 1|
|Gene Synonym(s)||AD3; Ad3h; Ad3h; FAD; PS-1; PS1; S182; alzheimer disease 3 homolog; presenilin-1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exon 7 was flanked by a single loxP site in intron 6 and floxed neo cassette in intron 7.|
|Mutations Made By|| |
Fred Van Leuven, Katholieke Universiteit Leuven
Mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for many generations to establish this congenic strain. When maintaining the live congenic colony, heterozygous or homozygous mice may be bred together.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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