These knock-in mice express a tamoxifen-inducible cre recombinase from the endogenous promoter/enhancer elements of the atonal homolog 1 (Drosophila) (Atoh1, also referred to as Math1) gene. When induced, cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordial. These mice may be useful in studying cerebellum development and fate mapping of Math1/Atoh1 cells.
Gord Fishell, Harvard Medical School
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Mice hemizygous for this Math1-CreERT2 transgene are viable and fertile. Under control of the Math1 (Atoh1) enhancer, tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Math1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Math1 expressing cells (the entire dorsal neural tube from posterior rhombomere 1 to the spinal cord); making them useful in studying cerebellum development and fate mapping of Math1/Atoh1 cells.
The Math1-CreERT2 transgene was designed with a 1.4 kb mouse Math1 (Atoh1) 3' enhancer (and a β-globin minimal promoter) sequence upstream of a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). The transgene was microinjected into FVB/N embryos. Founder mice were bred to mice on a mixed outbred background to test transgene efficacy. Mice found to exhibit appropriate expression levels and patterns (founder line 14) were selected and bred to Swiss Webster outbred mice. Transgenic mice were bred to Swiss Webster for several generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to FVB/NJ for at least one generation to establish the colony.
Expressed Gene | cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene, |
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Site of Expression | tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex) |
Allele Name | transgene insertion 14, Gordon Fishell |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Atoh1-cre; Math1-cre; Math1-CreERT2; Tg(Atoh1-cre/ESR1)1Fsh; Tg(HBB-cre)1Fsh |
Gene Symbol and Name | Tg(Atoh1-cre/Esr1*)14Fsh, transgene insertion 14, Gordon Fishell |
Gene Synonym(s) | |
Promoter | Atoh1, atonal bHLH transcription factor 1, mouse, laboratory |
Expressed Gene | cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene, |
Site of Expression | tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex) |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgene was designed with a 1.4 kb mouse Math1 (Atoh1) 3' enhancer (and a beta-globin minimal promoter) sequence upstream of a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). |
Mutations Made By | Rob Machold, The Skirball Institute, NYU Sch. of Med. |
When maintaining a live colony, transgenic mice can be bred to wildtype siblings or bred together, although a homozygous phenotype is not yet characterized (Aug-2007).
When using the Math1-CreERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #007684 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or wildtype for Tg(Atoh1-cre/Esr1*)14Fsh |
Frozen Mouse Embryo | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J Frozen Embryo | $3373.50 |
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