STOCK Esrrb^tm1.1Nat/J

Stock number: 007674
Product name: NR3B2^CKO
Research areas: Internal/Organ Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

These Nr3b2^CKO mutant mice may be useful in generating conditional mutations to study disorders of hearing and balance, inner ear development (such as endolymph-producing epithelia within the auditory and vestibular divisions), and control of cell fate decisions by nuclear receptors.

Detailed description

Mice homozygous for this Nr3b2^CKO allele possess loxP sites flanking exon 2 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exon containing the initiator methionine codon and encoding the N-terminal 132 amino acids (including part of the DNA-binding domain) deleted in the cre-expressing tissue(s). Of note, if the conditional Nr3b2^CKO is deleted by Cre recombinase in the placenta and embryo, embryonic lethality will result (placental defect). If the conditional Nr3b2^CKO is deleted by Cre recombinase only in the embryo, the resulting mice exhibit an inner ear defect (decreased endolymph production) resulting in deafness and defective balance. These Nr3b2^CKO mutant mice may be useful in generating conditional mutations to study disorders of hearing and balance, inner ear development (such as endolymph-producing epithelia within the auditory and vestibular divisions), and control of cell fate decisions by nuclear receptors.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of endolymph producing epithelial cells.

When bred to a strain expressing Cre recombinase in epiblast derived cells (see Stock No. 004783, 008454 for example), this mutant mouse strain may be useful in studies of endolymph producing epithelial cells.

Development

A targeting vector was designed to place a loxP site 184 bp upstream, as well as a loxP site 123 bp downstream of exon 2 of the targeted gene. An FRT-flanked PGK-neo cassette was also placed just downstream of the 3' loxP site. This construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6. The neomycin selection cassette was excised by crossing to mice constitutively expressing the Flp recombinase (unspecified genetic background, see Stock No. 003800). Mice harboring this Nr3b2^CKO conditional allele (and a single remaining FRT site) were bred together for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.

Allele

Symbol: Esrrb^tm1.1Nat
Name: targeted mutation 1.1, Jeremy Nathans
MGI accession ID: MGI:3720481
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: CKO^flp; Nr3b2^CKO
Marker symbol: Esrrb
Marker name: estrogen related receptor, beta
Marker synonyms: DFNB35; ERR beta-2; Err2; ERRb; ERRbeta2; ESRL2; Estrrb; NR3B2
Chromosome: 12
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was designed to place a loxp site 184 bp upstream, as well as a loxP site 123 bp downstream of exon 2 of the targeted gene. An FRT-flanked PGK-neo cassette was also placed just downstream of the 3' loxP site. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 (to generate Nr3b2^CKO+neo mice). The neomycin selection cassette was excised by crossing to mice constitutively expressing the Flp recombinase (unspecified genetic background, see Stock No. 003800), generating this Nr3b2^CKO allele with a single FRT site remaining.