B6.129S4-Pdgfra^{tm11(EGFP)Sor}/J

Stock number: 007669
Product name: PDGFRα^EGFP
Research areas: Cell Biology Research, Developmental Biology Research, Research Tools

Description

These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence patterns mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family).

Detailed description

Mice homozygous for this knock-in targeted mutation have an embryonic lethal phenotype, with half of the embryos failing to survive past embryonic day 12.5 and the remainder failing to survive beyond embryonic day 15.5. These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. Expression of H2BGFP mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family).

Development

A targeting vector containing a human H2B histone protein and enhanced Green Fluorescent Protein fusion gene, H2B-GFP or H2B-eGFP, and a floxed PGKneo selection cassette was used to disrupt sequence of the targeted gene that encodes the signal peptide and the first two immunoglobulin domains of the targeted gene. The endogenous Pdgfra promoter drives expression of the H2B-eGFP fusion gene. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice for 17 generations.

Allele

Symbol: Pdgfra^{tm11(EGFP)Sor}
Name: targeted mutation 11, Philippe Soriano
MGI accession ID: MGI:2663656
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Pdgfra
Marker name: platelet derived growth factor receptor, alpha polypeptide
Chromosome: 5
Strain of origin: 129S4/SvJaeSor
Expressed genes: GFP,Green Fluorescent Protein,; H2B-eGFP,Histone H2B-enhanced Green Fluorescent Protein,
Site of expression: Expression of the H2B-EGFP fusion protein mimicks the expression pattern of the endogenous Pdgfra gene. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm.
Molecular note: The gene was disrupted by a knock-in vector consisting of an enhanced GFP fused with an human Hist2h2be gene (H2Be-GFP) for chromatin localization and a floxed PGK-neo cassette.