Jumonji domain containing 6 targeted mutation homozygotes are defective in removing apoptotic cells, which accumulate in the lung and brain, causing abnormal development and neonatal lethality. Approximately 15% of the homozygotes manifest a hyperplastic brain phenotype resembling that of mice deficient in other cell death-associated genes. This strain may be useful in studies of mammalian organogenesis, respiratory distress syndromes and congenital brain malformations.
Dr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Del(11Jmjd6-Mettl23)1Flv | deletion, Chr 11, Richard A Flavell 1 |
Homozygotes are defective in removing apoptotic cells, which accumulate in the lung and brain, causing abnormal development and neonatal lethality. Approximately 15% of the homozygotes manifest a hyperplastic brain phenotype resembling that of mice deficient in other cell death-associated genes. The absence of expression in homozygous mice has been confirmed by Northern blot analysis of embryonic fibroblasts. This strain may be useful in studies of mammalian organogenesis, respiratory distress syndromes and congenital brain malformations.
Update: The targeting vector also replaced the first exon of the neighboring gene Mettl23, located on the opposite strand. RT-PCR confirmed absence of Mettl23 mRNA in homozygous embryos.
A targeting vector containing a loxP-flanked neomycin resistance gene was use to replace the first two exons of the gene. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. This strain has been backcrossed to C67BL/6 ten times by the donating laboratory.
Update: The targeting vector also replaced the first exon of the neighboring gene Mettl23, located on the opposite strand. RT-PCR confirmed absence of Mettl23 mRNA in homozygous embryos.
Allele Name | deletion, Chr 11, Richard A Flavell 1 |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Jmjd6tm1Flv; PSR - |
Gene Symbol and Name | Del(11Jmjd6-Mettl23)1Flv, deletion, Chr 11, Richard A Flavell 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | The first 2 exons were replaced with a neomycin resistance gene. Absence of expression after this gene modification was confirmed by Northern blot analysis. A subsequent publication demonstrated that the targeting vector also replaced the first exon of the neighboring gene Mettl23, located on the opposite strand. RT-PCR confirmed absence of Mettl23 mRNA in homozygous embryos. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintained as a live colony, heterozygous crosses may be used. Homozygotes die postnatally.
When using the B6.129S6-Del(11Jmjd6-Mettl23)1Flv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007666 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wildtype for Jmjd6<tm2Flv> |
Frozen Mouse Embryo | B6.129S6-Jmjd6<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Jmjd6<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Jmjd6<tm1Flv>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Jmjd6<tm1Flv>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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