B6.129S6-Del(11Jmjd6-Mettl23)1Flv/J

Stock number: 007666
Research areas: Apoptosis Research, Developmental Biology Research

Description

Jumonji domain containing 6 targeted mutation homozygotes are defective in removing apoptotic cells, which accumulate in the lung and brain, causing abnormal development and neonatal lethality. Approximately 15% of the homozygotes manifest a hyperplastic brain phenotype resembling that of mice deficient in other cell death-associated genes. This strain may be useful in studies of mammalian organogenesis, respiratory distress syndromes and congenital brain malformations.

Detailed description

Homozygotes are defective in removing apoptotic cells, which accumulate in the lung and brain, causing abnormal development and neonatal lethality. Approximately 15% of the homozygotes manifest a hyperplastic brain phenotype resembling that of mice deficient in other cell death-associated genes. The absence of expression in homozygous mice has been confirmed by Northern blot analysis of embryonic fibroblasts. This strain may be useful in studies of mammalian organogenesis, respiratory distress syndromes and congenital brain malformations.

Update: The targeting vector also replaced the first exon of the neighboring gene Mettl23, located on the opposite strand. RT-PCR confirmed absence of Mettl23 mRNA in homozygous embryos.

Development

A targeting vector containing a loxP-flanked neomycin resistance gene was use to replace the first two exons of the gene. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. This strain has been backcrossed to C57BL/6 ten times by the donating laboratory.

Update: The targeting vector also replaced the first exon of the neighboring gene Mettl23, located on the opposite strand. RT-PCR confirmed absence of Mettl23 mRNA in homozygous embryos.