Homozygous females are not fertile due to the failure of their reproductive tract development. Homozygous males are also infertile; although spermatogenesis and accessory structures appear unaffected, abnormalities in the neuronal control of penile erection were found. Null mice are notably smaller than their wildtype and heterozygous littermates several days after birth. A dramatic impairment of endochondral ossification and an attenuation of longitudinal vertebra or limb-bone growth are also seen in null animals. This strain may be useful in studies of bone, female reproductive tract development, and female/male fertility.
David L. Garbers, U.T. Southwestern Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Npr2 | natriuretic peptide receptor 2 |
Homozygous females are not fertile due to the failure of their reproductive tract development. Homozygous males are also infertile; although spermatogenesis and accessory structures appear unaffected, abnormalities in the neuronal control of penile erection have been found. Null mice are notably smaller than their wildtype and heterozygous littermates several days after birth. Heterozygous mice show a slight reduction in naso-anal length. A dramatic impairment of endochondral ossification and an attenuation of longitudinal vertebra or limb-bone growth are also seen in null animals. They show self-clasping and priapism, suggesting neuronal disorders, but no histological abnormalities are seen in the brain or spinal cord. Survival is reduced. Lethal tonic-clonic seizure attacks have been observed. mRNA is not detected in the brain, growth-plate cartilage, or primary cultures of dermal fibroblasts as determined by Northern blot analysis. This strain may be useful in studies of bone, female reproductive tract development, and female/male fertility.
A targeting vector was designed to replace exons 3-7, encoding the C-terminal half of the extracellular ligand-binding domain of the transmembrane segment, with a neomycin resistance gene. The vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted cells were injected into C57BL/6 blastocysts. Chimeric males with germline transmission were bred to C57BL/6 females. Records for this strain are not well defined; it is believed to be on a mixed background composed of C57BL/6, 129 and SJL.
Allele Name | targeted mutation 1, David L Garbers |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Npr2- |
Gene Symbol and Name | Npr2, natriuretic peptide receptor 2 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 4 |
Molecular Note | Exons 3-7, which encode the C-terminal half of the extracellular ligand-binding domain and the transmembrane segment, were replaced with a neo. mRNA was not detected in brains of mutant animals. |
Mutations Made By | David Garbers, U.T. Southwestern Medical Center |
When maintained as a live colony, heterozygotes may be bred. Both male and female homozygous mice are sterile.
When using the STOCK Npr2tm1Gar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007658 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Npr2<tm1Gar> |
Frozen Mouse Embryo | STOCK Npr2<tm1Gar>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Npr2<tm1Gar>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Npr2<tm1Gar>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Npr2<tm1Gar>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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