STOCK Npr2^tm1Gar/J

Stock number: 007658
Research areas: Developmental Biology Research, Reproductive Biology Research

Description

Homozygous females are not fertile due to the failure of their reproductive tract development. Homozygous males are also infertile; although spermatogenesis and accessory structures appear unaffected, abnormalities in the neuronal control of penile erection were found. Null mice are notably smaller than their wildtype and heterozygous littermates several days after birth. A dramatic impairment of endochondral ossification and an attenuation of longitudinal vertebra or limb-bone growth are also seen in null animals. This strain may be useful in studies of bone, female reproductive tract development, and female/male fertility.

Detailed description

Homozygous females are not fertile due to the failure of their reproductive tract development. Homozygous males are also infertile; although spermatogenesis and accessory structures appear unaffected, abnormalities in the neuronal control of penile erection have been found. Null mice are notably smaller than their wildtype and heterozygous littermates several days after birth. Heterozygous mice show a slight reduction in naso-anal length. A dramatic impairment of endochondral ossification and an attenuation of longitudinal vertebra or limb-bone growth are also seen in null animals. They show self-clasping and priapism, suggesting neuronal disorders, but no histological abnormalities are seen in the brain or spinal cord. Survival is reduced. Lethal tonic-clonic seizure attacks have been observed. mRNA is not detected in the brain, growth-plate cartilage, or primary cultures of dermal fibroblasts as determined by Northern blot analysis. This strain may be useful in studies of bone, female reproductive tract development, and female/male fertility.

Development

A targeting vector was designed to replace exons 3-7, encoding the C-terminal half of the extracellular ligand-binding domain of the transmembrane segment, with a neomycin resistance gene. The vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted cells were injected into C57BL/6 blastocysts. Chimeric males with germline transmission were bred to C57BL/6 females. Records for this strain are not well defined; it is believed to be on a mixed background composed of C57BL/6, 129 and SJL.

Allele

Symbol: Npr2^tm1Gar
Name: targeted mutation 1, David L Garbers
MGI accession ID: MGI:3522129
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Npr2
Marker name: natriuretic peptide receptor 2
Chromosome: 4
Strain of origin: 129S6/SvEvTac
Molecular note: Exons 3-7, which encode the C-terminal half of the extracellular ligand-binding domain and the transmembrane segment, were replaced with a neo. mRNA was not detected in brains of mutant animals.