Homozygous males are completely infertile. Sperm cells fail to acquire hyperactivated motility associated with penetration of the extracellular matrix of the egg. This strain may be useful in studies of male fertility.
David L. Garbers, U.T. Southwestern Medical Center
Homozygotes are viable. Males, but not females, are completely infertile. The targeted mutation fails to significantly alter sperm production, protein tyrosine phosphorylation associated with capacitation, induction of the acrosome reaction, forward velocity or percentage of motility. Sperm cells fail to acquire hyperactivated motility associated with penetration of the extracellular matrix of the egg. SDS/PAGE and immunoblotting of total sperm protein were used to confirm the absence of gene product. This strain may be useful in studies of male fertility.
A targeting vector was designed to replace exons 2-5 of the gene with a neomycin drug resistance cassette. The vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Chimeric mice were generated and backcrossed once to C57BL/6 to produce heterozygotes.
|Allele Name||targeted mutation 1, David L Garbers|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Catsper2, cation channel, sperm associated 2|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A neomycin selection cassette replaced a genomic fragment encompassing exons 2 through 5. The deleted region included the start codon and encoded the first three transmembrane helices. Protein was undetected in homozygous mutant mice by Western blot and immunocytochemical analyses of spermatozoa.|
|Mutations Made By|| |
David Garbers, U.T. Southwestern Medical Center
When maintained as a live colony, heterozygotes may be bred. Homozygous males, but not females are infertile.
When using the B6;129S6-Catsper2tm1Gar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007656 in your Materials and Methods section.