Tg(Thy1-COP4/EYFP)9Gfng (or Thy1-ChR2-YFP) transgenic mice may be useful in optogenetic studies for rapid control of motor behavior by addition or removal of light, for ex vivo and in vivo studies of neural circuitry/connectivity following illumination, and for fluorescent labeling of Thy1-expressing cells throughout the brain (including cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers and retinal ganglion cells).
Guoping Feng, Massachusetts Institute of Technology
Genetic Background | Generation |
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N6+N6F11
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$278.00 Domestic price for female 4-week |
348.51 Domestic price for breeder pair |
These Thy1-ChR2-YFP founder line 9 transgenic mice express the light-activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), fused to Yellow Fluorescent Protein (ChR2-YFP) under the control of the mouse thymus cell antigen 1 (Thy1) promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the transgenic ChR2-YFP fusion protein is detected throughout the brain, including in the cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers and retinal ganglion cells. Neurons expressing the transgene are morphologically and physiologically comparable to non-mutant neurons. This mutant mouse strain may be useful for ex vivo and in vivo neural circuitry mapping studies using light stimulation.
The ChR2-YFP fusion protein is composed of a Chlamydomonas reinhardtii-derived channelrhodopsin-2 (ChR2) fused in-frame with an enhanced yellow fluorescent protein (EYFP).
The bacterial opsins are retinal-binding proteins that combine a light-sensitive domain with an ion channel or pump; providing light-dependent ion transport, membrane potential alteration, and sensory functions to bacteria. ChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating ChR2-expressing neurons with blue light (~470 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells.
This optogenetic strain is one of many from the same transgene creator/donating investigator with light-inducible neurobiology applications; including
Thy1-ChR2-YFP line 18 (Stock No. 007612),
Thy1-eNpHR-YFP line 2 (Stock No. 012332),
Thy1-eNpHR-YFP line 4 (Stock No. 012334),
Thy1-vChR1-YFP line 1 (Stock No. 012341),
Thy1-vChR1-YFP line 4 (Stock No. 012344),
Thy1-vChR1-YFP line 8 (Stock No. 012348),
Thy1-mhChR2-YFP Line 20 (Stock No. 012350),
Prv-mhChR2-YFP Line 15 (Stock No. 012355),
ChAT-ChR2-YFP line 5 (Stock No. 014545),
ChAT-ChR2-YFP line 6 (Stock No. 014546),
VGAT-ChR2-YFP line 8 (Stock No. 014548),
and TpH2-ChR2-YFP line 5 (Stock No. 014555).
View YFP fluorescence in sagittal brain sections for this strain.
A transgenic construct containing Channelrhodopsin-2 from the green alga Chlamydomonas reinhardtii and Yellow Fluorescent Protein fusion gene under the control of the mouse thymus cell antigen 1 promoter was injected into C57BL/6 X SJL F1 hybrid donor eggs. Founder line 9 was subsequently established. The mice were backcrossed to C57BL/6 for six generations before arriving at The Jackson Laboratory.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Expressed Gene | COP4, Channelrhodopsin, Chlamydomonas |
Site of Expression | widespread expression in the brain including the cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers amd retinal ganglion cells; channelrhodopsin-2 and YFP |
Allele Name | transgene insertion 9, Guoping Feng |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | ChR2-YFP; Thy1-ChR2-YFP |
Gene Symbol and Name | Tg(Thy1-COP4/EYFP)9Gfng, transgene insertion 9, Guoping Feng |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | COP4, Channelrhodopsin, Chlamydomonas |
Site of Expression | widespread expression in the brain including the cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers amd retinal ganglion cells; channelrhodopsin-2 and YFP |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
General Note | Lines initiated from 7 of 10 transgenic founder mice exhibited expression of the chimeric protein in the central and/or peripheral nervous system, the specific pattern of expression varying among lines. |
Molecular Note | This transgene gives rise to a fusion protein composed of the first 315 amino acids of the light-activated, non-specific ion channel chlamyopsin 4 (COP4), also known as channelopsin 2 (CHOP2) and as channelrhodopsin 2 (ChR2), from the green alga Chlamydomonas reinhardtii joined to enhanced yellow fluorescent protein. The chimeric cDNA has replaced the Thy1 coding sequence and its associated introns, which contain sequences directing expression in non-neuronal cell types. The vector contains the entire Thy1 gene (including the essential promoter and neuron-specific upstream regulatory sequences, 3' polyA signal sequences and neuronal-specific expression signal containing introns) with the coding region removed (from the ATG in exon 2 to the end in exon 4, including intervening introns 2 and 3). Immunohistochemical analysis shows that mice with this transgene express the chimeric protein in neurons of several brain regions, including various nuclei of the brainstem, midbrain and thalamus; CA1 and CA3 pyramidal neurons of the hippocampus; strongly in layer 5 neurons of the cortex; and in retinal ganglion cells. |
When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.
When using the Thy1-ChR2-YFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #007615 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non-carrier for Tg(Thy1-COP4/EYFP)9Gfng |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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