These transgenic mice (“SLICK” for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons that express Yellow Fluorescent Protein. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.
Guoping Feng, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Reporter, Inducible) |
These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.
This strain is one of many from Dr. Guoping Feng (Duke University Medical Center) with fluorescent protein expression for neurobiological studies, including SLICK line A (Stock No. 007606), Thy1-ChR2-YFP line 18 (Stock No. 007612), and Thy1-ChR2-YFP line 9 (Stock No. 007615).
View YFP fluorescence in sagittal brain sections for this strain.
A transgenic construct containing the cre/ESR1 fusion gene (CreERT2), enhanced Yellow Fluorescent Protein under the control of 2 copies (placed back to back to function bidirectionally) of the mouse thymus cell antigen 1 promoter was microinjected into C57BL/6 X SJL F1 donor eggs. Founder line V was subsequently established. A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed this strain was on a mixed C57BL/6;SJL genetic background.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | tamoxifen-inducible Cre and YFP in many hippocampal dentate granule cells and in few neurons of the cortex, retinal ganglia, amygdala, brain stem and inferior and superior colliculi |
Allele Name | transgene insertion V, Guoping Feng |
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Allele Type | Transgenic (Recombinase-expressing, Reporter, Inducible) |
Allele Synonym(s) | SLICK; SLICK-V; Tg(Thy1-cre/ESR1,-EYFP)VGfng |
Gene Symbol and Name | Tg(Thy1-cre/ERT2,-EYFP)VGfng, transgene insertion V, Guoping Feng |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | tamoxifen-inducible Cre and YFP in many hippocampal dentate granule cells and in few neurons of the cortex, retinal ganglia, amygdala, brain stem and inferior and superior colliculi |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | Transgenic mice have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. |
Mutations Made By | Guoping Feng, Massachusetts Institute of Technology |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007610 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(Thy1-cre/ESR1,-EYFP)VGfng |
Frozen Mouse Embryo | B6;SJL-Tg(Thy1-cre/ERT2 -EYFP)VGfng/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Thy1-cre/ERT2 -EYFP)VGfng/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Thy1-cre/ERT2 -EYFP)VGfng/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(Thy1-cre/ERT2 -EYFP)VGfng/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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