B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J

Stock number: 007610
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice (“SLICK” for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons that express Yellow Fluorescent Protein. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.

Detailed description

These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.

This strain is one of many from Dr. Guoping Feng (Duke University Medical Center) with fluorescent protein expression for neurobiological studies, including SLICK line A (Stock No. 007606), Thy1-ChR2-YFP line 18 (Stock No. 007612), and Thy1-ChR2-YFP line 9 (Stock No. 007615).

View YFP fluorescence in sagittal brain sections for this strain.

Development

A transgenic construct containing the cre/ESR1 fusion gene (CreER^T2), enhanced Yellow Fluorescent Protein under the control of 2 copies (placed back to back to function bidirectionally) of the mouse thymus cell antigen 1 promoter was microinjected into C57BL/6 X SJL F1 donor eggs. Founder line V was subsequently established. A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed this strain was on a mixed C57BL/6;SJL genetic background.

Allele

Symbol: Tg(Thy1-cre/ERT2,-EYFP)VGfng
Name: transgene insertion V, Guoping Feng
MGI accession ID: MGI:3719909
Generation method: Transgenic
Attributes: Recombinase-expressing; Reporter; Inducible
Marker symbol: Tg(Thy1-cre/ERT2,-EYFP)VGfng
Marker name: transgene insertion V, Guoping Feng
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish; cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: tamoxifen-inducible Cre and YFP in many hippocampal dentate granule cells and in few neurons of the cortex, retinal ganglia, amygdala, brain stem and inferior and superior colliculi
Molecular note: Transgenic mice have a tamoxifen-inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter.