These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest. The Donating Investigator reports that founder line A mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons.
Guoping Feng, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Reporter, Inducible) |
These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 promoter is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line A (SLICK-A) mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons.
Further characterization [Heimer-McGinn and Young 2011 genesis 49:942] reports that SLICK-A mice have high YFP fluorescence in some hippocampal regions and cortical layer V, but sparse/dim labeling in other regions (such as the midbrain and brainstem). Fluorescence is less uniform, but this allows single-neuron labeling. SLICK-A mice exhibit significant cre-mediated recombination in the absence of tamoxifen. This "leaky" recombination in SLICK-A mice was prominent in areas that have bright YFP labeling (such as hippocampal CA1 and cortical layer V). Following tamoxifen-induction, efficient recombination is observed in the expected cell types, but with some inefficient recombination observed as well (including hippocampal CA3 and cortical layers II/III). This publication concluded that YFP and creERT2 expression levels are tightly coupled in SLICK-A mice, and that excessive levels of creERT2 must be avoided to achieve tight control of recombinase activity when using SLICK-A mice.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
This strain is one of many from the same transgene creator/donating investigator with fluorescent protein expression/neurobiological research applications, including:
SLICK line A (Stock No. 007606),
SLICK line V (Stock No. 007610),
SLICK line H (Stock No. 012708),
GAD67-GFP line 3 (Stock No. 007673),
Thy1-GFP line O (Stock No. 007919),
Thy1-YFP line G (line 17) (Stock No. 014130),
Thy1-CFP line I (Stock No. 014131),
Thy1-ChR2-YFP line 18 (Stock No. 007612),
Thy1-ChR2-YFP line 9 (Stock No. 007615),
Thy1-eNpHR-YFP line 2 (Stock No. 012332),
Thy1-eNpHR-YFP line 4 (Stock No. 012334),
Thy1-vChR1-YFP line 1 (Stock No. 012341),
Thy1-vChR1-YFP line 4 (Stock No. 012344),
Thy1-vChR1-YFP line 8 (Stock No. 012348),
Thy1-mhChR2-YFP line 20 (Stock No. 012350),
Prv-mhChR2-YFP line 15 (Stock No. 012355),
ChAT-ChR2-YFP line 5 (Stock No. 014545),
ChAT-ChR2-YFP line 6 (Stock No. 014546),
VGAT-ChR2-YFP line 8 (Stock No. 014548),
TpH2-ChR2-YFP line 5 (Stock No. 014555),
and R26-CAG-LSL-2XChETA-tdTomato reporter mice (Stock No. 017455).
A transgenic construct containing the cre/ESR1 fusion gene (CreERT2), enhanced Yellow Fluorescent Protein under the control of 2 copies (placed back to back to function bidirectionally) of the mouse thymus cell antigen 1 promoter was microinjected into C57BL/6 X SJL F1 donor eggs. Founder line A was subsequently established. The mice were backcrossed to C57BL/6 for 6 generations.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | tamoxifen-inducible Cre and YFP in subsets of motor neurons and dorsal root ganglion neurons, and in many neurons of the cortex, hippocampus (both CA1 and dentate granule cells) and cerebellar mossy fibers |
Allele Name | transgene insertion A, Guoping Feng |
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Allele Type | Transgenic (Recombinase-expressing, Reporter, Inducible) |
Allele Synonym(s) | SLICK line A; SlickA; Tg(Thy1-cre/ESR1,-EYFP)Agfng |
Gene Symbol and Name | Tg(Thy1-cre/ERT2,-EYFP)AGfng, transgene insertion A, Guoping Feng |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | tamoxifen-inducible Cre and YFP in subsets of motor neurons and dorsal root ganglion neurons, and in many neurons of the cortex, hippocampus (both CA1 and dentate granule cells) and cerebellar mossy fibers |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | Transgenic mice have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. |
Mutations Made By | Guoping Feng, Massachusetts Institute of Technology |
When maintaining a live colony, these mice may be bred as homozygotes.
When using the SLICK line A , SLICK-A mouse strain in a publication, please cite the originating article(s) and include JAX stock #007606 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(Thy1-cre/ESR1,-EYFP)AGfng |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-cre/ERT2 -EYFP)AGfng/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-cre/ERT2 -EYFP)AGfng/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-cre/ERT2 -EYFP)AGfng/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-cre/ERT2 -EYFP)AGfng/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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