Mice homozygous for this tetracycline regulatory cassette knockin into the neuropeptide Y gene show a 3-fold increase over constitutive levels of expression. Treatment of mice with doxycycline blunts transcription and mRNA and protein levels fall to approximately 20% of wild-type levels. Overexpression of the gene decreased sensitivity to kainate-induced seizures but treatment with doxycycline increased sensitivity.
Dr. Richard Palmiter, University of Washington
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Npy | neuropeptide Y |
Mice homozygous for this tetracycline regulatory cassette knockin are viable and fertile and do not display any gross physical or behavioral abnormalities. The mice overexpress the gene 3-fold in all brain cells where it is normally expressed. Treatment of mice with doxycycline blunts transcription and mRNA and protein levels fall to approximately 20% of wild-type levels. It takes approximately a week for protein levels to subsequently fall to wild-type levels, and six weeks to fall to 20% that of wild-type mice. Treatment of adults with doxycycline has little effect on body weight or feeding. Overexpression of the gene decreased sensitivity to kainate-induced seizures but treatment with doxycycline increased sensitivity.
A targeting vector was designed to introduce a doxycycline (Dox)-regulated cassette into exon 1, such that the gene is expressed until the mice are given Dox to block transcription. The cassette included a TK-Neo and Ura3 gene, flanked by a pair of loxP sites in addition to a tetracycline-controlled transcriptional activator (tTA) cassette. The vector was introduced to 129S4/SvJaeSor-derived AK18.1 embryonic stem (ES) cells. Mice carrying the mutation were bred with Mox2-Cre to delete the TK-Neo and Ura3 genes, and subsequently bred to remove Cre from the background. This line has been maintained on a mixed 129S4 and C57BL/6 background by the donating laboratory.
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
---|---|
Site of Expression |
Allele Name | targeted mutation 2, Richard D Palmiter |
---|---|
Allele Type | Targeted |
Allele Synonym(s) | Npytet |
Gene Symbol and Name | Npy, neuropeptide Y |
Gene Synonym(s) | |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 6 |
Molecular Note | Tetracycline activator and tet promoter targeted to first exon such that expression can be turned off with doxycycline. The TK-Neo and Ura3 genes from the tetracycline cassette were removed by breeding with Mox2-cre mice. In the absence of doxycyline, there is approximately 4-fold more mRNA and increased protein expression in the brain. Doxycycline treatment for 10 days reduces protein expression by about 50%. |
Mutations Made By | Dr. Richard Palmiter, University of Washington |
When maintaining a live colony, these mice are bred as heterozygotes or homozygotes.
When using the B6;129S4-Npytm2Rpa/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007585 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Npy<tm2Rpa> |
Frozen Mouse Embryo | B6;129S4-Npy<tm2Rpa>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Npy<tm2Rpa>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Npy<tm2Rpa>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Npy<tm2Rpa>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.