These "Fgfr2-flox" mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.
IMR Colony, The Jackson Laboratory
David Ornitz, Washington University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Fgfr2 | fibroblast growth factor receptor 2 |
Mice homozygous for this Fgfr2flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.
For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration.
When crossed to a strain expressing Cre recombinase in the neural tube, midbrain and dorsal spinal cord (see Stock No. 009107), this mutant mouse strain may be useful in studies of lacrimal gland development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Mutant mice on a mixed genetic background were sent to The Jackson Laboratory (as Stock No. 007569). Upon arrival, some mice were additionally backcrossed to C57BL/6J to generate this congenic strain (Stock No. 007579).
Allele Name | targeted mutation 1, David M Ornitz |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Fgfr2delta8-10lox; Fgfr2f; Fgfr2fl; Fgfr2flox; Fgfr2Lox; Fgfr2loxP |
Gene Symbol and Name | Fgfr2, fibroblast growth factor receptor 2 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 7 |
Molecular Note | Exons 8 through 10 were flanked by a single loxP site in intron 7 and an FRT-flanked neo cassette with a 3' loxP site in intron 10. |
Mutations Made By | David Ornitz, Washington University School of Medicine |
After arriving at The Jackson Laboratory on a mixed background, mutant mice were bred to wildtype C57BL/6J (Stock No. 000664) for 5-10 generations. The resulting backcrossed mice may be thereafter bred together as homozygotes.
When using the B6.129X1(Cg)-Fgfr2tm1Dor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007579 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fgfr2<tm1Dor> |
Frozen Mouse Embryo | B6.129X1(Cg)-Fgfr2<tm1Dor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1(Cg)-Fgfr2<tm1Dor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1(Cg)-Fgfr2<tm1Dor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1(Cg)-Fgfr2<tm1Dor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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