B6.129X1(Cg)-Fgfr2^tm1Dor/J

Stock number: 007579
Research areas: Developmental Biology Research, Research Tools

Description

These "Fgfr2-flox" mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.

Detailed description

Mice homozygous for this Fgfr2^flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.

For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration.

When crossed to a strain expressing Cre recombinase in the neural tube, midbrain and dorsal spinal cord (see Stock No. 009107), this mutant mouse strain may be useful in studies of lacrimal gland development.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

Mutant mice on a mixed genetic background were sent to The Jackson Laboratory (as Stock No. 007569). Upon arrival, some mice were additionally backcrossed to C57BL/6J to generate this congenic strain (Stock No. 007579).

Allele

Symbol: Fgfr2^tm1Dor
Name: targeted mutation 1, David M Ornitz
MGI accession ID: MGI:3044679
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Fgfr2
Marker name: fibroblast growth factor receptor 2
Chromosome: 7
Strain of origin: 129X1/SvJ
Molecular note: Exons 8 through 10 were flanked by a single loxP site in intron 7 and an FRT-flanked neo cassette with a 3' loxP site in intron 10.