These transgenic mice overexpress neuroglobin (Ngb) and GFP in multiple tissues, and are resistant to ischemia. When crossed to a mouse model of Alzheimer's disease, the disease phenotype is attenuated. They may be useful in studies of cerebral and myocardial ischemia, stroke, and neurodegenerative disease.
IMR Colony, The Jackson Laboratory
David Greenberg, Buck Institute for Age Research
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inserted expressed sequence) |
These transgenic mice express mouse neuroglobin and Enhanced Green Fluorescent Protein under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer. Western blot analysis detects increased NGB protein in heart brain of homozygotes. Transgenic mice have NGB-overexpressing neurons, astrocytes and endothelial cells in the cerebral cortex. The donating investigator reports that fluorescence is detected in all tissues. Experimentally induced ischemia in transgenic mice results in cerebral infarct volume reduction of approximately 30% and myocardial infarct volume reduction of approximately 25% when compared to wild-type. Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cerebral (CNS) and myocardial ischemia and stroke.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the mouse neuroglobin gene Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred BDF X CD1 donor eggs. Founder line 1 was subsequently established. Hemizygotes were intercrossed to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, some mice were backcrossed to C57BL/6J for at least five generations to generate this congenic strain.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Green fluorescence has been reported in all tissues tested |
Allele Name | transgene insertion 1, David Greenberg |
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Allele Type | Transgenic (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Ngb-Tg; Tg(ACTB-Ngb,-EGFP)1Dgrn; Tg(CAG-Ngb,-EGFP)1Dgrn |
Gene Symbol and Name | Tg(CAG-Ngb,-EGFP)1Dgrn, transgene insertion 1, David Greenberg |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Green fluorescence has been reported in all tissues tested |
Strain of Origin | (C57BL/6 x DBA/2)F1 x CD-1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing the mouse neuroglobin gene, Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin (ACTB) promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred (C57BL/6 x DBA/2)F1 x CD-1 donor eggs. Founder line 1 was subsequently established. Constitutive Ngb protein expression is increased in the brain and heart (in vascular endothelial cells) of transgenic mice, as shown by Western blot. Immunohistochemistry shows increased numbers of neurons, astrocytes and endothelial cells expressing Ngb protein. |
Mutations Made By | Eric Giegerich, Buck Institute for Age Research |
Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice.
When using the B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007575 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(CAG-Ngb,-EGFP)1Dgrn |
Frozen Mouse Embryo | B6.Cg-Tg(CAG-Ngb -EGFP)1Dgrn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(CAG-Ngb -EGFP)1Dgrn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(CAG-Ngb -EGFP)1Dgrn/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(CAG-Ngb -EGFP)1Dgrn/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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