B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J

Stock number: 007575
Research areas: Cardiovascular Research, Neurobiology Research, Research Tools

Description

These transgenic mice overexpress neuroglobin (Ngb) and GFP in multiple tissues, and are resistant to ischemia. When crossed to a mouse model of Alzheimer's disease, the disease phenotype is attenuated. They may be useful in studies of cerebral and myocardial ischemia, stroke, and neurodegenerative disease.

Detailed description

These transgenic mice express mouse neuroglobin and Enhanced Green Fluorescent Protein under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer. Western blot analysis detects increased NGB protein in heart brain of homozygotes. Transgenic mice have NGB-overexpressing neurons, astrocytes and endothelial cells in the cerebral cortex. The donating investigator reports that fluorescence is detected in all tissues. Experimentally induced ischemia in transgenic mice results in cerebral infarct volume reduction of approximately 30% and myocardial infarct volume reduction of approximately 25% when compared to wild-type. Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cerebral (CNS) and myocardial ischemia and stroke.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A transgenic construct containing the mouse neuroglobin gene Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred BDF X CD1 donor eggs. Founder line 1 was subsequently established. Hemizygotes were intercrossed to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, some mice were backcrossed to C57BL/6J for at least five generations to generate this congenic strain.

Allele

Symbol: Tg(CAG-Ngb,-EGFP)1Dgrn
Name: transgene insertion 1, David Greenberg
MGI accession ID: MGI:3714625
Generation method: Transgenic
Attributes: Reporter; Inserted expressed sequence
Marker symbol: Tg(CAG-Ngb,-EGFP)1Dgrn
Marker name: transgene insertion 1, David Greenberg
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1 x CD-1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Green fluorescence has been reported in all tissues tested
Molecular note: A transgenic construct containing the mouse neuroglobin gene, Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin (ACTB) promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred (C57BL/6 x DBA/2)F1 x CD-1 donor eggs. Founder line 1 was subsequently established. Constitutive Ngb protein expression is increased in the brain and heart (in vascular endothelial cells) of transgenic mice, as shown by Western blot. Immunohistochemistry shows increased numbers of neurons, astrocytes and endothelial cells expressing Ngb protein.