Similar to mice of strain NOD.B6-(D6Mit254-D6Mit339)/CarJ (Stock #4482), these mutant mice carry a C57BL/6-derived congenic interval on Chromosome 6 which contains the Nk1.1 locus but is shorter and mice have a higher incidence of diabetes.
T1DR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Marker Symbol | Marker Name | |
---|---|---|
Klrb1c | killer cell lectin-like receptor subfamily B member 1C | |
D6Mit254 | DNA segment, Chr 6, Massachusetts Institute of Technology 254 | |
D6Mit289 | DNA segment, Chr 6, Massachusetts Institute of Technology 289 |
Klrb1ca (Nk1.1) homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Nk1.1 expression in the strain has been confirmed by FACS analysis of peripheral blood cells. The C57BL/6-derived congenic interval on Chromosome 6 which contains the Nk1.1 locus is shorter in these mice than that carried in the original strain (Stock No. 004482). Diabetes incidence in this strain is higher than that reported in strain 004482 (65% in females and 15% in males by 27 weeks for this strain versus 30% in the females and 0% males by 30 weeks of age for Stock No. 004482). Histological examination of the pancreas from 27-week old, non-diabetic mice reveals extensive insulitis that is not significantly different from that seen in similarly aged Stock No. 004482 mice or NOD/ShiLtJ inbred mice (Stock No. 001976). This model is useful for looking at the role of natural killer cells in autoimmune diseases such as Type 1 Diabetes.
A Chromosome 6 genomic segment from C57BL/6JOrl originally reported to span D6Mit254 through D6Mit14 and containing Klrb1ca (commonly referred to as NK1.1) was transferred through 10 generations of backcrossing to NOD/Necker (Carnaud et al, 2001). In 2002, this strain arrived at The Jackson Laboratory at N10F? (Stock No. 004482). A partial genome scan indicated that the C57BL/JOrl congenic interval on Chr. 6 was longer than what was originally published. Stock No. 004482 was further backcrossed to NOD/ShiLtJ (Stock No. 001976) prior to making the stock homozygous for a smaller C57BL/6JOrl-derived interval (D6Mit254-D6Mit289) containing Klrb1ca [NK1.1 (62.1 cM)]. The C57BL/6 genetic segment in this NOD congenic stock is approximately 8.5cM shorter than the original stock received. In 2007, the generation of this strain is N10+N5F4.
When using the NOD.NK1.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #007573 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous, 1 pair minimum |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit289)/CarJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit289)/CarJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit289)/CarJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit289)/CarJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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