STOCK Fgfr2^tm1Dor/J

Stock number: 007569
Research areas: Developmental Biology Research, Research Tools

Description

These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.

Detailed description

Mice homozygous for this Fgfr2^flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.

For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration.

When crossed to a strain expressing Cre recombinase in the neural tube, midbrain and dorsal spinal cord (see Stock No. 009107), this mutant mouse strain may be useful in studies of lacrimal gland development.

Development

A targeting vector was designed to insert a loxp site in intron 7 and an FRT-neo cassette and loxP site in intron 10 of the targeted gene. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6. Mice harboring this "Fgfr2-flox" mutant allele were bred to other mutant mice (C57 and 129 genetic backgrounds). Mice were then bred to C57BL/6J for 4-7 generations (to remove the unwanted mutations and then make the strain congenic). Mice harboring only the Fgfr2-flox mutant allele were sent to The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed background (5 out of 27 markers are segregating for 129 genetic background).

Allele

Symbol: Fgfr2^tm1Dor
Name: targeted mutation 1, David M Ornitz
MGI accession ID: MGI:3044679
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Fgfr2
Marker name: fibroblast growth factor receptor 2
Chromosome: 7
Strain of origin: 129X1/SvJ
Molecular note: Exons 8 through 10 were flanked by a single loxP site in intron 7 and an FRT-flanked neo cassette with a 3' loxP site in intron 10.