Overview

Also Known As:CD11c-Cre-GFP line 4097

These CD11c-Cre-GFP transgenic mice may be useful in immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.

Donating Investigator

Alexander Chervonsky, The University of Chicago

Genetic overview

Genetic Background	Generation
	N?+pN10F18 (2020-04-13 00:00:00)

Tg(Itgax-cre,-EGFP)4097Ach

Allele Type
Transgenic (Recombinase-expressing)

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

333.51 Domestic price for breeder pair

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Details

Detailed Description

Mice hemizygous for this CD11c-Cre-GFP transgene are viable and fertile. The CD11c (Itgax) promoter directs bicistronic Cre and EGFP protein expression to dendritic cells (DCs). Expression of EGFP is expected to have equimolar expression with Cre recombinase. When bred with any mouse containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence. These CD11c-Cre-GFP transgenic mice, as well as CD11c-Cre transgenic mice (see Stock No. 008068), may be useful for immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.

Development

The CD11c-Cre-GFP transgene was designed placing a Cre recombinase gene, internal ribosomal entry site (IRES), and an enhanced green fluorescent protein (EGFP) gene all downstream of the 5.3 kb dendritic cell (DC)-specific mouse integrin alpha X (Itgax/Cd11c) promoter/enhancer. This transgene was microinjected into C57BL/6J fertilized mouse embryos, which were then implanted into pseudopregnant foster mice. Transgenic mice were bred to C57BL/6J, and the resulting transgenic offspring (founder line 4097) were established. The donating investigator reports that this transgene inserted on chromosome 13. CD11c-Cre-GFP transgenic line 4097 was maintained by breeding with C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory in 2008. Upon arrival, CD11c-Cre-GFP transgenic line 4097 was backcrossed with C57BL/6J inbred mice (Stock No. 000664). By March 2014, the colony had been backcrossed for 10 generations. Thereafter, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) or C57BL/6J mice.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	EGFP and Cre recombinase are expressed in dendritic cells (DCs).

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Stranges PB; Watson J; Cooper CJ; Choisy-Rossi CM; Stonebraker AC; Beighton RA; Hartig H; Sundberg JP; Servick S; Kaufmann G; Fink PJ; Chervonsky AV. 2007. Elimination of antigen-presenting cells and autoreactive T cells by Fas contributes to prevention of autoimmunity. Immunity 26(5):629-41PubMed: 17509906 MGI: J:123556

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Genetics

Tg(Itgax-cre,-EGFP)4097Ach

Allele Symbol: Tg(Itgax-cre,-EGFP)4097Ach

Allele Name	transgene insertion 4097, Alexander V Chervonsky
Allele Type	Transgenic (Recombinase-expressing)
Allele Synonym(s)	CD11c-Cre; CD11c-Cre 4907; CD11c-Cre-GFP; CD11c-Cre-GFP.4097; Tg(Itgax-cre,EGFP)4097Ach
Gene Symbol and Name	Tg(Itgax-cre,-EGFP)4097Ach, transgene insertion 4097, Alexander V Chervonsky
Gene Synonym(s)
Promoter	Itgax, integrin alpha X, mouse, laboratory
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	EGFP and Cre recombinase are expressed in dendritic cells (DCs).
Strain of Origin	C57BL/6J
Chromosome	UN
General Note	Two lines were created with line 4097 exhibiting greater expression than 4272 in dendritic cells.
Molecular Note	The Itgax promoter and enchancer were used to drive cre expression in a pIRES2-EGFP-based plasmid. Two lines were created with line 4097 exhibiting greater expression than 4272 in dendritic cells.
Mutations Made By	Alexander Chervonsky, The University of Chicago

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
- Immunology, Inflammation and Autoimmunity Research
- Fluorescent Proteins
Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers

Mammalian Phenotype Terms by Genotype

References

Stranges PB; Watson J; Cooper CJ; Choisy-Rossi CM; Stonebraker AC; Beighton RA; Hartig H; Sundberg JP; Servick S; Kaufmann G; Fink PJ; Chervonsky AV. 2007. Elimination of antigen-presenting cells and autoreactive T cells by Fas contributes to prevention of autoimmunity. Immunity 26(5):629-41PubMed: 17509906 MGI: J:123556

Additional References

Brocker T; Riedinger M; Karjalainen K. 1997. Targeted expression of major histocompatibility complex (MHC) class II molecules demonstrates that dendritic cells can induce negative but not positive selection of thymocytes in vivo. J Exp Med 185(3):541-50PubMed: 9053454 MGI: J:38890

Additional - Tg(Itgax-cre,-EGFP)4097Ach related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Tg(Itgax-cre,-EGFP)4097Ach Probe
- QPCR:Tg(Itgax-cre,-EGFP)4097Ach
- Standard PCR:Tg(Itgax-cre)
- Standard PCR:Fluorescent Proteins (Generic GFP)
- Standard PCR:Tg(Itgax-cre,-EGFP)4097Ach
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

From 2008-2014, CD11c-Cre-GFP transgenic line 4097 was backcrossed with C57BL/6J inbred mice (Stock No. 000664). By March 2014, the colony had been backcrossed for 10 generations. Thereafter, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) or C57BL/6J mice.
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
- Hemizygote x Noncarrier
Citation
When using the CD11c-Cre-GFP line 4097 mouse strain in a publication, please cite the originating article(s) and include JAX stock #007567 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Hemizygous or Non Carrier for Tg(Itgax-cre,-EGFP)4097Ach

Related Products and Services

Frozen Mouse Embryo	C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:CD11c-Cre-GFP line 4097

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Itgax-cre,-EGFP)4097Ach

Allele Symbol: Tg(Itgax-cre,-EGFP)4097Ach

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Tg(Itgax-cre,-EGFP)4097Ach related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information