C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J

Stock number: 007567
Product name: CD11c-Cre-GFP line 4097
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These CD11c-Cre-GFP transgenic mice may be useful in immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.

Detailed description

Mice hemizygous for this CD11c-Cre-GFP transgene are viable and fertile. The CD11c (Itgax) promoter directs bicistronic Cre and EGFP protein expression to dendritic cells (DCs). Expression of EGFP is expected to have equimolar expression with Cre recombinase. When bred with any mouse containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence. These CD11c-Cre-GFP transgenic mice, as well as CD11c-Cre transgenic mice (see Stock No. 008068), may be useful for immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.

Development

The CD11c-Cre-GFP transgene was designed placing a Cre recombinase gene, internal ribosomal entry site (IRES), and an enhanced green fluorescent protein (EGFP) gene all downstream of the 5.3 kb dendritic cell (DC)-specific mouse integrin alpha X (Itgax/Cd11c) promoter/enhancer. This transgene was microinjected into C57BL/6J fertilized mouse embryos, which were then implanted into pseudopregnant foster mice. Transgenic mice were bred to C57BL/6J, and the resulting transgenic offspring (founder line 4097) were established. The donating investigator reports that this transgene inserted on chromosome 13. CD11c-Cre-GFP transgenic line 4097 was maintained by breeding with C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory in 2008. Upon arrival, CD11c-Cre-GFP transgenic line 4097 was backcrossed with C57BL/6J inbred mice (Stock No. 000664). By March 2014, the colony had been backcrossed for 10 generations. Thereafter, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) or C57BL/6J mice.

Allele

Symbol: Tg(Itgax-cre,-EGFP)4097Ach
Name: transgene insertion 4097, Alexander V Chervonsky
MGI accession ID: MGI:3720729
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Itgax-cre,-EGFP)4097Ach
Marker name: transgene insertion 4097, Alexander V Chervonsky
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: cre,cre recombinase,bacteriophage P1; GFP,Green Fluorescent Protein,
Site of expression: EGFP and Cre recombinase are expressed in dendritic cells (DCs).
Molecular note: The Itgax promoter and enchancer were used to drive cre expression in a pIRES2-EGFP-based plasmid. Two lines were created with line 4097 exhibiting greater expression than 4272 in dendritic cells.
General note: Two lines were created with line 4097 exhibiting greater expression than 4272 in dendritic cells.