These CD11c-Cre-GFP transgenic mice may be useful in immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.
Alexander Chervonsky, The University of Chicago
Genetic Background | Generation |
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N?+pN10F18
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Mice hemizygous for this CD11c-Cre-GFP transgene are viable and fertile. The CD11c (Itgax) promoter directs bicistronic Cre and EGFP protein expression to dendritic cells (DCs). Expression of EGFP is expected to have equimolar expression with Cre recombinase. When bred with any mouse containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence. These CD11c-Cre-GFP transgenic mice, as well as CD11c-Cre transgenic mice (see Stock No. 008068), may be useful for immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.
The CD11c-Cre-GFP transgene was designed placing a Cre recombinase gene, internal ribosomal entry site (IRES), and an enhanced green fluorescent protein (EGFP) gene all downstream of the 5.3 kb dendritic cell (DC)-specific mouse integrin alpha X (Itgax/Cd11c) promoter/enhancer. This transgene was microinjected into C57BL/6J fertilized mouse embryos, which were then implanted into pseudopregnant foster mice. Transgenic mice were bred to C57BL/6J, and the resulting transgenic offspring (founder line 4097) were established. The donating investigator reports that this transgene inserted on chromosome 13. CD11c-Cre-GFP transgenic line 4097 was maintained by breeding with C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory in 2008. Upon arrival, CD11c-Cre-GFP transgenic line 4097 was backcrossed with C57BL/6J inbred mice (Stock No. 000664). By March 2014, the colony had been backcrossed for 10 generations. Thereafter, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) or C57BL/6J mice.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP and Cre recombinase are expressed in dendritic cells (DCs). |
Allele Name | transgene insertion 4097, Alexander V Chervonsky |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | CD11c-Cre; CD11c-Cre 4907; CD11c-Cre-GFP; CD11c-Cre-GFP.4097; Tg(Itgax-cre,EGFP)4097Ach |
Gene Symbol and Name | Tg(Itgax-cre,-EGFP)4097Ach, transgene insertion 4097, Alexander V Chervonsky |
Gene Synonym(s) | |
Promoter | Itgax, integrin alpha X, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP and Cre recombinase are expressed in dendritic cells (DCs). |
Strain of Origin | C57BL/6J |
Chromosome | UN |
General Note | Two lines were created with line 4097 exhibiting greater expression than 4272 in dendritic cells. |
Molecular Note | The Itgax promoter and enchancer were used to drive cre expression in a pIRES2-EGFP-based plasmid. Two lines were created with line 4097 exhibiting greater expression than 4272 in dendritic cells. |
Mutations Made By | Alexander Chervonsky, The University of Chicago |
From 2008-2014, CD11c-Cre-GFP transgenic line 4097 was backcrossed with C57BL/6J inbred mice (Stock No. 000664). By March 2014, the colony had been backcrossed for 10 generations. Thereafter, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) or C57BL/6J mice.
When using the CD11c-Cre-GFP line 4097 mouse strain in a publication, please cite the originating article(s) and include JAX stock #007567 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizyous or Non Carrir for Tg(Itgax-cre,-EGFP)4097Ach |
Frozen Mouse Embryo | C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(Itgax-cre -EGFP)4097Ach/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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