Overview

Also Known As: MOR-

These Oprm1^tm1Kff (or MOR-) knock-out mice exhibit a lack of morphine analgesia, reward, and withdrawal. These knock-out mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.

Donating Investigator

Brigitte L. Kieffer, McGill University

Genetic overview

Genetic Background	Generation
	N12+N2F5 (2018-12-11 00:00:00)

Oprm1^tm1Kff

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Oprm1	opioid receptor, mu 1

View Genetics

Research Applications

Neurobiology Research
Sensorineural Research
Research Tools

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female

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Details

Detailed Description

Mice homozygous for the mu-opioid receptor mutant allele Oprm1^tm1Kff (or MOR-) are viable and fertile. MOR-selective ligand binding is absent on brain membranes from homozygous mice. Homozygous mice exhibit a lack of morphine analgesia, reward, and withdrawal. This is accompanied by decreased mechanical, thermal, and chemical pain thresholds. Homozygous mice also show decreased ethanol self-administration and decreased THC-conditioned place aversion. In contrast to mutant mice deficient of delta- or kappa-opioid receptors (Stock Nos. 007557 or 007558, respectively), Oprm1^tm1Kff homozygotes exhibit hypolocomotive spontaneous stress responses. Indeed, the reduced anxiety and depressive-like behavior observed in Oprm1^tm1Kff mutants is in stark contrast to kappa-opioid receptor deficient mice. These knock-out mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These mutant mice were originally published on a mixed genetic background. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to insert a PGK-neo cassette into exon 2 of the targeted gene. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were bred with C57BL/6 mice. These mu-opioid receptor (MOR) mutant mice were backcrossed to C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Martin M; Matifas A; Maldonado R; Kieffer BL. 2003. Acute antinociceptive responses in single and combinatorial opioid receptor knockout mice: distinct mu, delta and kappa tones. Eur J Neurosci 17(4):701-8PubMed: 12603260 MGI: J:108005
Matthes HW; Maldonado R; Simonin F; Valverde O; Slowe S; Kitchen I; Befort K; Dierich A; Le Meur M; Dolle P; Tzavara E; Hanoune J; Roques BP; Kieffer BL. 1996. Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the mu-opioid-receptor gene [see comments] Nature 383(6603):819-23PubMed: 8893006 MGI: J:36241

View All References

Genetics

Oprm1^tm1Kff

Allele Symbol: Oprm1^tm1Kff

Allele Name	targeted mutation 1, Brigitte L Kieffer
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Brigitte L Kieffer; Oprm1^tm1Kff
Gene Symbol and Name	Oprm1, opioid receptor, mu 1
Gene Synonym(s)	LMOR; muOR; MORA; MOP; MOP receptor; MOP-R; MOR; MOR-1; MOR1; M-OR-1; OPRM; Oprrm1; Oprm; mor
Strain of Origin	129S2/SvPas
Chromosome	10
Molecular Note	A neomycin selection cassette was inserted into exon 2. Binding assays on brain of homozygous animals confirmed that no functional protein was made from this allele.
Mutations Made By	Brigitte Kieffer, McGill University

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

autism spectrum disorder

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Behavioral and Learning Defects
  - genes regulating preferences to alcohol and stress
  - genes regulating preferences to alcohol
  - mu opioid receptor deficiency
- Receptor Defects
  - opiod
Sensorineural Research
- Nociception
Research Tools
- Toxicology Research
  - drug/compound testing
- Sensorineural Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Oprm1^tm1Kff/Oprm1^tm1Kff
involves: 129S2/SvPas

behavior/neurological phenotype

abnormal pain threshold
- administration of morphine does not affect threshold response in tail immersion and hot plate tests
- attenuated response after foot-shock exposure

decreased behavioral withdrawal response
- prolonged morphine administration does not create symptoms of dependence or withdrawal

hypoactivity
- mild change

impaired behavioral response to anesthetic
- in a tail flick assay, mice treated with morphine-6beta-glucuronide (M6G) or 6-acetyl-morphine exhibit no analgesic response unlike similarly treated wild-type mice

impaired behavioral response to morphine
- Normal - however, loperamine-induced analgesia is maintained
- in a tail flick assay, mice treated with morphine-6beta-glucuronide (M6G) or 6-acetyl-morphine exhibit no analgesic response unlike similarly treated wild-type mice
- morphine-induced analgesia is abolished
- no affect on conditioned place preference induced by morphine
- Normal - no effect on conditioned place preference induced by morphine
- prolonged morphine administration does not create symptoms of dependence or withdrawal

increased coping response
- slightly decreased immobility time in forced-swim test

integument phenotype

abnormal pain threshold
- administration of morphine does not affect threshold response in tail immersion and hot plate tests
- attenuated response after foot-shock exposure

Genotype: Oprm1^tm1Kff/Oprm1^tm1Kff
involves: 129S2/SvPas * C57BL/6J

behavior/neurological phenotype

abnormal social investigation
- males do not show a change in social exploratory activity when exposed to playback of female ultrasonic vocalizations unlike wild-type males that exhibit an increase of exploratory activity
- Normal - males however, exhibit normal behavior in the auditory cued-fear conditioning task

References

Martin M; Matifas A; Maldonado R; Kieffer BL. 2003. Acute antinociceptive responses in single and combinatorial opioid receptor knockout mice: distinct mu, delta and kappa tones. Eur J Neurosci 17(4):701-8PubMed: 12603260 MGI: J:108005
Matthes HW; Maldonado R; Simonin F; Valverde O; Slowe S; Kitchen I; Befort K; Dierich A; Le Meur M; Dolle P; Tzavara E; Hanoune J; Roques BP; Kieffer BL. 1996. Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the mu-opioid-receptor gene [see comments] Nature 383(6603):819-23PubMed: 8893006 MGI: J:36241

Additional - Oprm1^tm1Kff related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Oprm1^tm1Kff
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous or homozygous mice may be bred.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the MOR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #007559 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

FGB29 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Oprm1<tm1Kff>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6.129S2-Oprm1<tm1Kff>/J	$2595.00
Frozen Mouse Embryo	B6.129S2-Oprm1<tm1Kff>/J	$2595.00
Frozen Mouse Embryo	B6.129S2-Oprm1<tm1Kff>/J	$3373.50
Frozen Mouse Embryo	B6.129S2-Oprm1<tm1Kff>/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

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Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: MOR-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Oprm1tm1Kff

Allele Symbol: Oprm1tm1Kff

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Oprm1tm1Kff/Oprm1tm1Kffinvolves: 129S2/SvPas

behavior/neurological phenotype

integument phenotype

Genotype: Oprm1tm1Kff/Oprm1tm1Kffinvolves: 129S2/SvPas * C57BL/6J

behavior/neurological phenotype

References

Additional - Oprm1tm1Kff related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Oprm1^tm1Kff

Allele Symbol: Oprm1^tm1Kff

Genotype: Oprm1^tm1Kff/Oprm1^tm1Kff
involves: 129S2/SvPas

Genotype: Oprm1^tm1Kff/Oprm1^tm1Kff
involves: 129S2/SvPas * C57BL/6J

Additional - Oprm1^tm1Kff related