These Oprm1tm1Kff (or MOR-) knock-out mice exhibit a lack of morphine analgesia, reward, and withdrawal. These knock-out mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.
Brigitte L. Kieffer, McGill University
Genetic Background | Generation |
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N12+N2F5
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Oprm1 | opioid receptor, mu 1 |
Mice homozygous for the mu-opioid receptor mutant allele Oprm1tm1Kff (or MOR-) are viable and fertile. MOR-selective ligand binding is absent on brain membranes from homozygous mice. Homozygous mice exhibit a lack of morphine analgesia, reward, and withdrawal. This is accompanied by decreased mechanical, thermal, and chemical pain thresholds. Homozygous mice also show decreased ethanol self-administration and decreased THC-conditioned place aversion. In contrast to mutant mice deficient of delta- or kappa-opioid receptors (Stock Nos. 007557 or 007558, respectively), Oprm1tm1Kff homozygotes exhibit hypolocomotive spontaneous stress responses. Indeed, the reduced anxiety and depressive-like behavior observed in Oprm1tm1Kff mutants is in stark contrast to kappa-opioid receptor deficient mice. These knock-out mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These mutant mice were originally published on a mixed genetic background. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to insert a PGK-neo cassette into exon 2 of the targeted gene. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were bred with C57BL/6 mice. These mu-opioid receptor (MOR) mutant mice were backcrossed to C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Allele Name | targeted mutation 1, Brigitte L Kieffer |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | MOR- |
Gene Symbol and Name | Oprm1, opioid receptor, mu 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 10 |
Molecular Note | A neomycin selection cassette was inserted into exon 2. Binding assays on brain of homozygous animals confirmed that no functional protein was made from this allele. |
Mutations Made By | Brigitte Kieffer, McGill University |
When maintaining a live colony, heterozygous or homozygous mice may be bred.
When using the MOR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #007559 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Oprm1<tm1Kff> |
Frozen Mouse Embryo | B6.129S2-Oprm1<tm1Kff>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Oprm1<tm1Kff>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Oprm1<tm1Kff>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Oprm1<tm1Kff>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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