These A kinase (PRKA) anchor protein 6 (Akap6)-mutant mice may be useful in studying developmental biology and craniofacial defects.
John D. Scott, University of Washington
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Akap6 | A kinase (PRKA) anchor protein 6 |
Homozygous mice have reduced viability, especially on a C57BL/6 background. Approximately 1 out of 4 homozygotes are very small and die before reaching 2 weeks of age. The remaining homozygotes are 20-30% smaller than wildtype and heterozygous littermates; these survive and eventually become similar in size to wildtypes. All homozygotes have a characteristic craniofacial defect that includes a very pointed nose, eyes that are delayed in opening, and a shortened jaw. The alpha transcript of the gene is absent, as determined by brain mRNA and protein assays. Beta transcript RNA is present in heart and brain.
A targeting vector was designed to replace exon 2 with an IRES, LacZ, URA and neomycin cassette and thus inhibit the production of the alpha alternative transcript. The vector was injected into 129-derived embryonic stem (ES) cells. This line (line T38) has been backcrossed to C57BL/6 for at least 10 generations.
Allele Name | targeted mutation 1, John D Scott |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | mAKAP alpha- |
Gene Symbol and Name | Akap6, A kinase (PRKA) anchor protein 6 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 12 |
Molecular Note | A targeting vector was designed to replace exon 2 with an IRES, LacZ, URA and neomycin cassette and thus inhibit the production of the alpha alternative transcript. |
Mutations Made By | John Scott, University of Washington |
When maintained as a live colony, heterozygotes may be bred. Homozygotes have reduced viability.
When using the B6.129-Akap6tm1Jsco/38J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007448 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Akap6-<tm1Jsco> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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