Homozygous mutants exhibit morphological and functional defects of the central nervous system with reduced spine density in the hippocampus and cortex, and mild impairment in memory retention.
John D. Scott, University of Washington
Homozygotes are viable, healthy and normal in size. The level of mutant protein expressed by homozygous mice is similar to that of wildtype animals as determined by immunoprecipitation of brain extracts, but the level of protein binding to Rac GTPase-activating protein (WRP; formally SRGAP3) is reduced by 50%. The mice have reduced spine density in CA1 of the hippocampus and layer of the cortex. There is also an elevated late-phase long-term potentiation (L-LTP) and mild impairment in memory retention.
A targeting vector was designed to place a neomycin cassette in intron 6, and delete amino acids 322-332 and 425-431 of exon 7. The vector was introduced to 129X1/SvJ-derived JSI-1 embryonic stem (ES) cells. Chimeric mice were generated and backcrossed to C57BL/6 for at least 10 generations by the donating laboratory.
|Allele Name||targeted mutation 2, John D Scott|
|Allele Type||Targeted (Hypomorph)|
|Allele Synonym(s)||wave m|
|Gene Symbol and Name||Wasf1, WAS protein family, member 1|
|Promoter||Wasf1, WAS protein family, member 1, mouse, laboratory|
|Strain of Origin||129X1/SvJ|
|General Note||ES cell line = JSI-1.|
|Molecular Note||A targeting vector was designed to place a neomycin cassette in intron 6, and delete amino acids 322-332 and 425-431 of exon 7. Protein levels are similar to wild-type as shown by immunoprecipitation, but the level of protein binding to Rac GTPase-activating protein (WRP) is reduced by 50%.|
|Mutations Made By|| |
John Scott, University of Washington
When maintained as a live colony, heterozygotes may be crossed with wildtype or C57BL/6J mice.
When using the mWAVE-1 KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #007446 in your Materials and Methods section.