B6.129X1-Wasf1^tm2Jsco/J

Stock number: 007446
Product name: mWAVE-1 KI
Research areas: Neurobiology Research

Description

Homozygous mutants exhibit morphological and functional defects of the central nervous system with reduced spine density in the hippocampus and cortex, and mild impairment in memory retention.

Detailed description

Homozygotes are viable, healthy and normal in size. The level of mutant protein expressed by homozygous mice is similar to that of wildtype animals as determined by immunoprecipitation of brain extracts, but the level of protein binding to Rac GTPase-activating protein (WRP; formally SRGAP3) is reduced by 50%. The mice have reduced spine density in CA1 of the hippocampus and layer of the cortex. There is also an elevated late-phase long-term potentiation (L-LTP) and mild impairment in memory retention.

Development

A targeting vector was designed to place a neomycin cassette in intron 6, and delete amino acids 322-332 and 425-431 of exon 7. The vector was introduced to 129X1/SvJ-derived JSI-1 embryonic stem (ES) cells. Chimeric mice were generated and backcrossed to C57BL/6 for at least 10 generations by the donating laboratory.

Allele

Symbol: Wasf1^tm2Jsco
Name: targeted mutation 2, John D Scott
MGI accession ID: MGI:3714530
Generation method: Targeted
Attributes: Hypomorph
Marker symbol: Wasf1
Marker name: WAS protein family, member 1
Chromosome: 10
Strain of origin: 129X1/SvJ
Molecular note: A targeting vector was designed to place a neomycin cassette in intron 6, and delete amino acids 322-332 and 425-431 of exon 7. Protein levels are similar to wild-type as shown by immunoprecipitation, but the level of protein binding to Rac GTPase-activating protein (WRP) is reduced by 50%.
General note: ES cell line = JSI-1.