Homozygous knockout mutants exhibit increased mortality induced by gamma-irradiation, increased B cell derived lymphoma incidence, splenomegaly, and premature death.
Andrei Gudkov, Cleveland Clinic Foundation
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ing1 | inhibitor of growth family, member 1 |
Mice homozygous for the targeted mutation are viable, fertile, and do not display any gross physical or behavioral abnormalities. Homozygotes are smaller than their wildtype littermates. Homozygous births from heterozygous crosses occur at a lower than expected frequency. During a 23 month period homozygotes exhibit increased morbidity or visible abnormalities including splenomegaly and an increased incidence of lymphomas (follicular center B-cell lymphomas) when compared to wild type littermates. When exposed to 6 consecutive daily doses of 2.34Gy total body gamma irradiation, mutant mice have a lower survival rate than wildtype controls. Mouse embryonic fibroblasts (MEFs) from homozygous animals are slightly more sensitive to UV-B exposure. This mutant mouse strain may be useful in studies of hypersensitivity to gamma irradiation and tumorigenesis.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4. The construct was electroporated into 129S1/Sv-p+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygotes were bred to generate homozygotes. The mice were then backcrossed to C57BL/6 for 11 generations prior to their arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, A V Gudkov |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ing1- |
Gene Symbol and Name | Ing1, inhibitor of growth family, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 8 |
Molecular Note | A targeting vector was designed to replace exon 4, common to all transcripts and containing most of the protein coding sequences including the conserved PHD finger domain, with a neomycin resistance gene. Transcript was not detected in mutant MEFS in Northern blot analysis. Western blot failed to detect protein in mutants as well. |
Mutations Made By | Julia Kichina, Cleveland Clinic Foundation |
When maintaining a live colony, these mice are bred as homozygotes. The donating investigator used a homozygous X homozygous breeding scheme. Female homozygous mice may neglect or destroy litters.
When using the Ing1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007263 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ing1<tm1Avg> |
Frozen Mouse Embryo | B6.129S1-Ing1<tm1Avg>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Ing1<tm1Avg>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Ing1<tm1Avg>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Ing1<tm1Avg>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.