C57BL/6J and C57BL/6JEi show greater sensitivity to sex reversal alleles than do other mouse strains and this sensitivity is increased by the consomic substitution of the Y Chromosome from specific inbred strains that have a particular Sry sequence defined by Washburn et al. (Genetics 1989 and 2001). The inbred strains with this variant Sry sequence include AKR/J, LEWES/Ei, MA/MyJ, PL/J, RF/J, and WSB/Ei. Thus, the C57BL/6J-Chr YAKR/J/EiJ consomic strain is prone to sex reversal when additionally made haploinsufficient for a gene important in male lineage development, which includes but is not limited to Zfpm2, Gata4, Map3k4, and Nr0b1. (Bouma et al., 2007; Bogani et al., 2009; Bouma et al., 2005.)
The AKR/J Y Chromosome was moved onto C57BL/6JEi through repeated backcrossing. In 2007 sperm were cryopreserved from males at generation N73.
|Allele Name||sex-determining region of Chr Y- AKR/J|
|Allele Synonym(s)||AKR YDOM; YAKR|
|Gene Symbol and Name||Sry, sex determining region of Chr Y|
|Strain of Origin||AKR/J|
|Molecular Note||This allele contains all the information necessary and sufficient to initiate normal testis development but is conditionally dysfunctional on the C57BL/6 background.|