B6.129(FVB)-Col1a2^tm1.1Mcbr/J

Stock number: 007248
Research areas: Cell Biology Research, Developmental Biology Research

Description

This strain has been discontinued.

Please consider a related strain (Stock No. 001815). Alternatively, please search the JAX Mice database for other strains which may be suitable for your studies or contact Technical Support for assistance.

Detailed description

These mice harbor a point mutation knock-in patterned after the variant found among Old Order Amish kindred (OOA) of Lancaster County, Pennsylvania. Mice exhibit reduced body mass and reduced bone mass density by 2 months of age. Homozygotes do not survive to weaning age. These mutant mice express mutant type I collagen similar to that observed in humans with osteogenesis imperfecta. Incorporation of the point mutation was verified by sequence analysis. Mutant mRNA is detected by RT-PCR analysis of total RNA.

Development

A targeting vector containing a loxP flanked neomycin resistance cassette was used to introduce a point mutation equivalent to the human G610C mutation, into exon 33 (Note: mouse exon 33 is equivalent to human exon 35). The construct was electroporated into 129/SvEv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting chimeric animals were bred to C57BL/6J. Male animals were then crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J female mice (Stock No. 003724) to remove the selection cassette. The mice were backcrossed to C57BL/6J for 4 generations before arriving at The Jackson Laboratory. Upon arrival the mice were backcrossed to C57BL/6J for 1 generation.

Allele

Symbol: Col1a2^tm1.1Mcbr
Name: targeted mutation 1.1, Daniel J McBride
MGI accession ID: MGI:3711122
Generation method: Targeted
Attributes: Inserted expressed sequence
Marker symbol: Col1a2
Marker name: collagen, type I, alpha 2
Chromosome: 6
Strain of origin: 129/SvEv
Molecular note: A floxed neo cassette was inserted upstream of exon 35 and a G to T mutation was inserted in exon 35 resulting in a glycine to cysteine mutation at codon 706. Cre mediated recombination removed the neo cassette.