Mice homozygous for this knock-out gene exhibit defective splenic B-cell maturation and impaired T-cell-dependent antibody formation.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnfrsf13c | tumor necrosis factor receptor superfamily, member 13c |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or FACS analysis of spleen tissue and cells, respectively. Homozygotes have reduced numbers of mature recirculating bone marrow and splenic B cells. B cell development is arrested between the transitional IgM+ (T1 + T2) and IgMlow (T3) stages. Homozygotes exhibit diminished antigen-specific antibody responses with decreased levels of IgM, IgG1, IgG2, IgG2b and IgG3. This mutant mouse strain may be useful in studies of B cell development and differentiation.
A targeting vector was used to insert loxP sites on either side of exons 3 and 4, and a FRT site flanked neomycin resistance cassette. The construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c recipient blastocysts. The donating investigator reported that all mice were maintained on a pure C57BL/6 background (see SNP note below). The resulting chimeric animals were crossed to C57BL/6 transgenic mice expressing Cre recombinase under the control of the cytomegalovirus promoter, to remove the floxed exons. The donating investigator reports that the mice do not carry the cre transgene.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1, Marc Schmidt-Supprian |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Baff-r-; BAFFR- |
Gene Symbol and Name | Tnfrsf13c, tumor necrosis factor receptor superfamily, member 13c |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 15 |
Molecular Note | Cre-recombinase excised a floxed region containing exons 3 and 4. The excised region encoded the entire extracellular and transmembrane domains and part of the cytoplasmic domain. RT-PCR did not detect mRNA in mutants. FACS analysis showed no surface expression of mutant splenic cells. |
Mutations Made By | Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine |
When maintaining a live colony, these mice may be bred as homozygotes.
When using the BAFF-R KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007212 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tnfrsf13c<tm1Mass> |
Frozen Mouse Embryo | B6(Cg)-Tnfrsf13c<tm1Mass>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tnfrsf13c<tm1Mass>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tnfrsf13c<tm1Mass>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Tnfrsf13c<tm1Mass>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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