B6(Cg)-Tnfrsf13c^tm1Mass/J

Stock number: 007212
Product name: BAFF-R KO
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Mice homozygous for this knock-out gene exhibit defective splenic B-cell maturation and impaired T-cell-dependent antibody formation.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or FACS analysis of spleen tissue and cells, respectively. Homozygotes have reduced numbers of mature recirculating bone marrow and splenic B cells. B cell development is arrested between the transitional IgM+ (T1 + T2) and IgM^low (T3) stages. Homozygotes exhibit diminished antigen-specific antibody responses with decreased levels of IgM, IgG1, IgG2, IgG2b and IgG3. This mutant mouse strain may be useful in studies of B cell development and differentiation.

Development

A targeting vector was used to insert loxP sites on either side of exons 3 and 4, and a FRT site flanked neomycin resistance cassette. The construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c recipient blastocysts. The donating investigator reported that all mice were maintained on a pure C57BL/6 background (see SNP note below). The resulting chimeric animals were crossed to C57BL/6 transgenic mice expressing Cre recombinase under the control of the cytomegalovirus promoter, to remove the floxed exons. The donating investigator reports that the mice do not carry the cre transgene.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Tnfrsf13c^tm1Mass
Name: targeted mutation 1, Marc Schmidt-Supprian
MGI accession ID: MGI:3054891
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tnfrsf13c
Marker name: tumor necrosis factor receptor superfamily, member 13c
Chromosome: 15
Strain of origin: B6.Cg-Thy1^a
Molecular note: Cre-recombinase excised a floxed region containing exons 3 and 4. The excised region encoded the entire extracellular and transmembrane domains and part of the cytoplasmic domain. RT-PCR did not detect mRNA in mutants. FACS analysis showed no surface expression of mutant splenic cells.