B6;129S4-Plekha1^{Gt(ROSA)82Sor}/J

Stock number: 007201
Research areas: Cell Biology Research, Developmental Biology Research, Reproductive Biology Research

Description

These Plekha1-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation. Homozygous males are infertile and, after 3 weeks of age, exhibit higher than normal (wildtype) weight gain.

Detailed description

25% of homozygotes die by 2 weeks of age. Homozygous males are infertile and, after 3 weeks of age, exhibit higher than normal (wildtype) weight gain. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. These Plekha1-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.

Development

The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAβgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Plekha1 gene (in the 3rd intron) were identified and used to generate mutant mice. Heterozygous mice were maintained on a mixed B6;129 background prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.

The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.

Allele

Symbol: Plekha1^{Gt(ROSA)82Sor}
Name: gene trapped 82, Philippe Soriano
MGI accession ID: MGI:3526857
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Synonyms: Plekha1^-
Marker symbol: Plekha1
Marker name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Chromosome: 7
Strain of origin: 129S4/SvJaeSor
Molecular note: The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was inserted into intron 3.