These Plekha1-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation. Homozygous males are infertile and, after 3 weeks of age, exhibit higher than normal (wildtype) weight gain.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Null/Knockout) | Plekha1 | pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1 |
25% of homozygotes die by 2 weeks of age. Homozygous males are infertile and, after 3 weeks of age, exhibit higher than normal (wildtype) weight gain. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. These Plekha1-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAβgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Plekha1 gene (in the 3rd intron) were identified and used to generate mutant mice. Heterozygous mice were maintained on a mixed B6;129 background prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.
The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.
Allele Name | gene trapped 82, Philippe Soriano |
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Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | Plekha1- |
Gene Symbol and Name | Plekha1, pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 7 |
Molecular Note | The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was inserted into intron 3. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
When maintaining a live colony, these mice can be bred as heterozygotes. 25% of homozygotes die by 2 weeks of age. Homozygote males are infertile. The Donating Investigator used a heterozygous male x homozygous female breeding scheme.
When using the B6;129S4-Plekha1Gt(ROSA)82Sor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007201 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Plekha1<Gt(ROSA)82Sor> |
Frozen Mouse Embryo | B6;129S4-Plekha1<Gt(ROSA)82Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Plekha1<Gt(ROSA)82Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Plekha1<Gt(ROSA)82Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Plekha1<Gt(ROSA)82Sor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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