B, T, and myeloid cells in mice homozygous for this targeted point mutation are hyperresponsive to stimulation, and B cell development is skewed to the B1 cell lineage. A mild lymphoproliferative disorder is developed but mice develop low titer or no autoantibodies.
Dr. Arthur Weiss, University of California, San Francisco
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Humanized sequence) | Ptprc | protein tyrosine phosphatase, receptor type, C |
Mice homozygous for this targeted point mutation are viable and fertile. The phenotype of this mutation, which inactivates the inhibitory wedge of the protein, is dependent upon the genetic background. On the 129S6 background, B, T, and myeloid cells are hyperresponsive to stimulation at a biochemical and cellular level. B cell development is skewed to the B1 cell lineage. A mild lymphoproliferative disorder develops, but the autoantibodies associated with the mixed C57BL/6 and 129 background do not develop (or do so at low titer). Cell surface expression of the protein is indistinguishable from wildtype as determined by flow cytometry of hematpoietic lineages in bone marrow, lymph node, spleen, thymus, peritoneal lavage, and peripheral blood.
A targeting vector carrying the glutamate 613 (E613R) mutation in exon 18 and a loxP-flanked neomycin resistance cassette in the downstream intron was transfected into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Targeted clones were microinjected into C57BL/6 blastocysts and mice derived from the resultant lines were bred to a C57BL/6 background Actb-Cre transgenic mouse to eliminate the neomycin resistance cassette. This line was crossed to C57BL/6, and later backcrossed to 129S6/SvEvTac for nine generations by the donating laboratory.
Allele Name | targeted mutation 1, Arthur Weiss |
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Allele Type | Targeted (Humanized sequence) |
Allele Synonym(s) | CD45 E613R; Cd45w; Wedge |
Gene Symbol and Name | Ptprc, protein tyrosine phosphatase, receptor type, C |
Gene Synonym(s) | |
Promoter | Ptprc, protein tyrosine phosphatase, receptor type, C, mouse, laboratory |
Strain of Origin | 129X1/SvJ |
Chromosome | 1 |
Molecular Note | To recapitulate a mutation in the orthologous human gene that disrupts the inhibitory wedge, a glutamate to arginine substitution at codon 613 (E613R) was engineered in exon 18 and incorporated at the endogenous locus via homolgous recombination. A single loxP site remained in intron 18 after the floxed neo cassette was excised. |
Mutations Made By | Dr. Arthur Weiss, University of California, San Francisco |
When maintaining a live colony, these mice are bred as heterozygotes or homozygotes.
When using the CD45 E613R mouse strain in a publication, please cite the originating article(s) and include JAX stock #007172 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Ptprc<tm1Weis> |
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