B6.FVB(Cg)-Mmp9^tm1Tvu/J

Stock number: 007084
Product name: MMP-9^-
Research areas: Cancer Research, Cardiovascular Research, Developmental Biology Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Sensorineural Research

Description

Because Mmp9^tm1Tvu homozygous mice show altered inflammatory responses they may be used to study injury response and repair, angiogenesis and inflammatory response.

Detailed description

Mice that are homozygous null for the Mmp9 (matrix metalloproteinase 9) gene are viable and fertile. No Mmp9 activity is detected in spleen cell lysates. Long bones (tibia, femur) are 10% shorter in homozygous null mice. Histological examination of three-week-old mice reveals a dramatically lengthened zone of hypertrophic cartilage (six to eight times larger vs. wild-type) due to delayed apoptosis, vascularization, and ossification. Subsequent remodeling resolves the condition, resulting in normal appearing bones by eight weeks of age. Null mice show altered responses to repair of injury in skin, cornea, central nervous system and bone marrow reconstitution, and altered inflammatory responses.
Coat color expected from breeding is nonagouti. Of note, beginning ~mid-2021, The Jackson Laboratory live colony shows some homozygotes exhibit white belly spots in several pedigree lines throughout the colony. Because our colony is maintained by breeding homozygotes together, is not known if heterozygous mice have these spots [February 2023].

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was used to disrupt a most of exon 2 and all of intron 2 of the Mmp9 gene. The construct was electroporated into 129S-derived ZW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were mated with Swiss Black females. Progeny animals were mated to Black Swiss mice for an unknown number of generations before being mated with FVB/N animals. Upon arrival at The Jackson Laboratory mice, were mated to C57BL/6J animals for a minimum of N5 generations.

Allele

Symbol: Mmp9^tm1Tvu
Name: targeted mutation 1, Thiennu H Vu
MGI accession ID: MGI:1932294
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Mmp9
Marker name: matrix metallopeptidase 9
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: Part of exon 2 and all of intron 2 were replaced with a cassette containing the neomycin resistance gene driven by a PGK promoter.
General note: ES cell line = ZW4.