Because Mmp9tm1Tvu homozygous mice show altered inflammatory responses they may be used to study injury response and repair, angiogenesis and inflammatory response.
IMR Colony, The Jackson Laboratory
Mice that are homozygous null for the Mmp9 (matrix metalloproteinase 9) gene are viable and fertile. No Mmp9 activity is detected in spleen cell lysates. Long bones (tibia, femur) are 10% shorter in homozygous null mice. Histological examination of three-week-old mice reveals a dramatically lengthened zone of hypertrophic cartilage (six to eight times larger vs. wild-type) due to delayed apoptosis, vascularization, and ossification. Subsequent remodeling resolves the condition, resulting in normal appearing bones by eight weeks of age. Null mice show altered responses to repair of injury in skin, cornea, central nervous system and bone marrow reconstitution, and altered inflammatory responses.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was used to disrupt a most of exon 2 and all of intron 2 of the Mmp9 gene. The construct was electroporated into 129S-derived ZW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were mated with Swiss Black females. Progeny animals were mated to Black Swiss mice for an unknown number of generations before being mated with FVB/N animals. Upon arrival at The Jackson Laboratory mice, were mated to C57BL/6J animals for a minimum of N5 generations.
|Allele Name||targeted mutation 1, Thiennu H Vu|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Gelatinase B-Null; GelB-; Mmp9-; MMP-9 KO; MMP-9-; MMP9-; MMP-9KO|
|Gene Symbol and Name||Mmp9, matrix metallopeptidase 9|
|Strain of Origin||129S6/SvEvTac|
|General Note||ES cell line = ZW4.|
|Molecular Note||Part of exon 2 and all of intron 2 were replaced with a cassette containing the neomycin resistance gene driven by a PGK promoter.|
|Mutations Made By|| |
Zena Werb, University of California, San Francisco
This strain originated on a B6;129 background, was mated to Black Swiss mice for an unknown number of generations and crossed to FVB/N mice for five generations. It then was crossed to C57BL/6J mice for at least five generations. Coat color expected from breeding:nonagouti
When using the MMP-9- mouse strain in a publication, please cite the originating article(s) and include JAX stock #007084 in your Materials and Methods section.