UBC-GFP transgenic mice express green fluorescent protein directed by the human ubiqutin C promoter, and were discovered to have transgene insertion on chromosome 17 resulting in linkage to H-2b MHC haplotype (see details below). Certain hematopoietic cell types display distinct expression levels of GFP, allowing identification of different cells types by FACS analysis. This strain is a useful tool for studying hematopoietic cell differentiation and in vivo leukocyte tracking. This transgene is also available on C57BL/6J as Stock No. 004353.
In 2020, the UBC-GFP transgene was determined to have integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and is closely linked to H-2b MHC haplotype. See Important Note for additional details.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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N6+N6F13
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Allele Type |
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Transgenic (Reporter) |
Allele Type | Gene Symbol | Gene Name |
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Not Applicable | H2 | histocompatibility-2, MHC |
Starting at:
$278.00 Domestic price for female 4-week |
$2,854.50 Domestic price Cryo Recovery |
Important Note regarding transgene insertion and linkage to H-2b MHC haplotype: In Liu et al. 2020 J Immunol. 204:1982 [PMID:32122998], it was discovered that the UBC-GFP transgene integrated into chromosome 17 at nucleotide position 29,435,589 (reference sequence NC_000083.6; GRCm38.p6) - this is a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1). The number of transgene copies was not determined. Furthermore, this determined that BALB/cBy-congenic UBC-GFP transgenic mice (N11+ from Stock No. 007076) retained the H-2b MHC haplotype from the genetic background upon which it was created (C57BL/6), rather than the H-2d MHC haplotype of BALB/cBy.
As of June 2020, all live breeder mice in the Stock No. 007076 colony genotyped as homozygous for H-2b and negative for H-2d.
In July-August 2020, a cohort of homozygous mice were confirmed to have H-2 Db and H-2 Kb expression in peripheral blood, similar to C57BL/6 [view data; July-August 2020].
Although the UBC-GFP transgene inserted close to the H-2 locus, segregation is possible. For experiments where MHC haplotype is a factor, researchers should determine if the UBC-GFP transgenic mice are H-2b or H-2d.
In 2020, the UBC-GFP transgene was determined to have integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and is closely linked to H-2b MHC haplotype. See Important Note for additional details.
These UBC-GFP transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the human ubiqutin C promoter.
Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express EGFP in all tissues examined. Certain hematopoietic cell types display distinct expression levels of EGFP, allowing identification of different cells types by FACS analysis. EGFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher EGFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoietic cell differentiation and in vivo tracking of leukocytes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing an enhanced green fluorescent protein (EGFP) open reading frame under the control of the human ubiqutin C promoter was microinjected into fertilized C57BL/6 oocytes. Mice were screened for genomic integration of the transgene by PCR and for GFP expression in peripheral blood leukocytes. The resulting female mouse exhibiting the desired phenotype was bred to a male C57BL/6J mouse. F1 progeny from this cross that expressed GFP were intercrossed to produce homozygotes. The mice were then backcrossed to BALB/cBy for 5 generations before arriving at The Jackson Laboratory.
In 2020, the UBC-GFP transgene was determined to have integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and is closely linked to H-2b MHC haplotype. See Important Note for additional details.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 17, was segregating.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development. |
Site of Expression |
Allele Name | transgene insertion 30, Brian Schaefer |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(UBCGFP)30Scha; UBC-GFP; Ub-GFP; UBI-EGFP; UBI-GFP; uGFP |
Gene Symbol and Name | Tg(UBC-GFP)30Scha, transgene insertion 30, Brian Schaefer |
Gene Synonym(s) | |
Promoter | UBC, ubiquitin C, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development. |
Strain of Origin | C57BL/6 |
Chromosome | 17 |
General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoietic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice. |
Molecular Note | The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. The transgene integrated into chromosome 17 at nucleotide position 29,435,589 (reference sequence NC_000083.6; GRCm38.p6) - this is a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H-2 K1). |
Mutations Made By | Brian Schaefer, Uniformed Services Univ. Health Sciences |
Allele Name | b variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | |
Gene Symbol and Name | H2, histocompatibility-2, MHC |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 17 |
General Note | The b variant has been observed in the following strains: C57BL/10, C57BL/10SnJ, BXSB/MpJ, C57BL/6J, C57L/J, LP/J, 129P3/J, 129P1/ReJ. |
When maintaining a live colony, homozygous mice may be bred together.
When using the UBI-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #007076 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(UBC-GFP)30Scha |
Frozen Mouse Embryo | CByJ.B6-Tg(UBC-GFP)30Scha/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | CByJ.B6-Tg(UBC-GFP)30Scha/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | CByJ.B6-Tg(UBC-GFP)30Scha/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | CByJ.B6-Tg(UBC-GFP)30Scha/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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