CByJ.129S2(B6)-Cd8a^tm1Mak/J

Stock number: 007071
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for the Cd8a^tm1Mak targeted mutation may be useful in studies of susceptibility to viral infections and the role of cytotoxic T-cells in immune response.

Detailed description

Mice homozygous for the Cd8a^tm1Mak targeted mutation are deficient in functional cytotoxic T-cells; however, helper T-cell development and function is comparable to normal.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing neomycin resistance gene was used to disrupt exon 1. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts.

The mice were backcrossed to C57BL/6 for 13 generations before arriving at The Jackson Laboratory. The mice were then backcrossed to BALB/cByJ for 5 generations (using a speed congenic protocol) before being made homozygous.

Allele

Symbol: Cd8a^tm1Mak
Name: targeted mutation 1, Tak Mak
MGI accession ID: MGI:1857149
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd8a
Marker name: CD8 antigen, alpha chain
Chromosome: 6
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance gene was inserted into exon 1. Flow cytometry analysis on thymus and lymph node cells derived from homozygous mice confirmed that no detectable encoded protein was expressed on the cell surface.