Mice homozygous for the Cd8atm1Mak targeted mutation may be useful in studies of susceptibility to viral infections and the role of cytotoxic T-cells in immune response.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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001026 BALB/cByJ |
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cd8a | CD8 antigen, alpha chain |
Mice homozygous for the Cd8atm1Mak targeted mutation are deficient in functional cytotoxic T-cells; however, helper T-cell development and function is comparable to normal.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing neomycin resistance gene was used to disrupt exon 1. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into recipient blastocysts.
The mice were backcrossed to C57BL/6 for 13 generations before arriving at The Jackson Laboratory.
The mice were then backcrossed to BALB/cByJ for 5 generations (using a speed congenic protocol) before being made homozygous.
Allele Name | targeted mutation 1, Tak Mak |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD8-; CD8 KO; CD8alpha-; CD8alpha-; Cd8atm1Mak; CD8KO; CD8-KO; Lyt-2- |
Gene Symbol and Name | Cd8a, CD8 antigen, alpha chain |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 6 |
Molecular Note | A neomycin resistance gene was inserted into exon 1. Flow cytometry analysis on thymus and lymph node cells derived from homozygous mice confirmed that no detectable encoded protein was expressed on the cell surface. |
Mutations Made By | Dr. Tak Mak, University Health Network/Un of Toronto |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the CByJ.129S2(B6)-Cd8atm1Mak/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007071 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildytpe for Cd8a<tm1Mak> |
Frozen Mouse Embryo | CByJ.129S2(B6)-Cd8a<tm1Mak>/J | $2595.00 |
Frozen Mouse Embryo | CByJ.129S2(B6)-Cd8a<tm1Mak>/J | $2595.00 |
Frozen Mouse Embryo | CByJ.129S2(B6)-Cd8a<tm1Mak>/J | $3373.50 |
Frozen Mouse Embryo | CByJ.129S2(B6)-Cd8a<tm1Mak>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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