Mice homozygous for the Cd8atm1Mak targeted mutation may be useful in studies of susceptibility to viral infections and the role of cytotoxic T-cells in immune response.
IMR Colony, The Jackson Laboratory
Mice homozygous for the Cd8atm1Mak targeted mutation are deficient in functional cytotoxic T-cells; however, helper T-cell development and function is comparable to normal.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing neomycin resistance gene was used to disrupt exon 1. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into recipient blastocysts.
The mice were backcrossed to C57BL/6 for 13 generations before arriving at The Jackson Laboratory.
The mice were then backcrossed to BALB/cByJ for 5 generations (using a speed congenic protocol) before being made homozygous.
|Allele Name||targeted mutation 1, Tak Mak|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD8-; CD8 KO; CD8alpha-; CD8alpha-; Cd8atm1Mak; CD8KO; CD8-KO; Lyt-2-|
|Gene Symbol and Name||Cd8a, CD8 antigen, alpha chain|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance gene was inserted into exon 1. Flow cytometry analysis on thymus and lymph node cells derived from homozygous mice confirmed that no detectable encoded protein was expressed on the cell surface.|
|Mutations Made By|| |
Dr. Tak Mak, University Health Network/Un of Toronto
When maintaining a live colony, these mice can be bred as homozygotes.
When using the CByJ.129S2(B6)-Cd8atm1Mak/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007071 in your Materials and Methods section.