B6.129-Crx^tm1Clc/J

Stock number: 007064
Product name: Crx KO
Research areas: Neurobiology Research, Sensorineural Research

Description

Homozygotes for this knock-out mutation exhibit a lack of photoreceptor outer segments and rod and cone activity, and circadian entrainment activity is attenuated.

Detailed description

Homozygotes are viable and fertile and do not display any gross physical abnormalities. These mice do not elaborate photoreceptor outer segments and synaptic termini, and they lack cone and rod activities as assayed by electroretinograms. Circadian entrainment activity is attenuated. Protein is not produced as determined by RT-PCR of retinal RNA extracts. This strain may be useful as a model of retinophathy (e.g., Leber's congenital amaurosis (LCA), cone-rod dystrophy-2 (adCRD2), retinitis pigmentosa (RP)), or circadian rhythm modification.

Development

A targeting vector was designed to replace exon 3 and a portion of exon 4 with a PGK-Neo cassette in reverse orientation. The vector was electroporated into a 129S2/SvEvTac-derived TC-1 embryonic stem (ES) cell line. Correctly-targeted ES cells were microinjected into C57BL/6 blastocysts. Resultant chimeric foundation mice were backcrossed to C57BL/6 at least 10 times.

Allele

Symbol: Crx^tm1Clc
Name: targeted mutation 1, Constance L Cepko
MGI accession ID: MGI:2181191
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Crx
Marker name: cone-rod homeobox
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: The homeodomain coding region containing exon 3 and a portion of exon 4 was replaced by a neomycin selection cassette. RT-PCR analysis showed an absence of transcript in the retinas of homozygous mutant mice.