Homozygotes for this knock-out mutation exhibit a lack of photoreceptor outer segments and rod and cone activity, and circadian entrainment activity is attenuated.
Connie Cepko, Harvard Medical School, HHMI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Crx | cone-rod homeobox |
Homozygotes are viable and fertile and do not display any gross physical abnormalities. These mice do not elaborate photoreceptor outer segments and synaptic termini, and they lack cone and rod activities as assayed by electroretinograms. Circadian entrainment activity is attenuated. Protein is not produced as determined by RT-PCR of retinal RNA extracts. This strain may be useful as a model of retinophathy (e.g., Leber's congenital amaurosis (LCA), cone-rod dystrophy-2 (adCRD2), retinitis pigmentosa (RP)), or circadian rhythm modification.
A targeting vector was designed to replace exon 3 and a portion of exon 4 with a PGK-Neo cassette in reverse orientation. The vector was electroporated into a 129S2/SvEvTac-derived TC-1 embryonic stem (ES) cell line. Correctly-targeted ES cells were microinjected into C57BL/6 blastocysts. Resultant chimeric foundation mice were backcrossed to C57BL/6 at least 10 times.
Allele Name | targeted mutation 1, Constance L Cepko |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Crx- |
Gene Symbol and Name | Crx, cone-rod homeobox |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | The homeodomain coding region containing exon 3 and a portion of exon 4 was replaced by a neomycin selection cassette. RT-PCR analysis showed an absence of transcript in the retinas of homozygous mutant mice. |
Mutations Made By | Connie Cepko, Harvard Medical School, HHMI |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the Crx KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007064 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Crx<tm1Clc> |
Frozen Mouse Embryo | B6.129-Crx<tm1Clc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Crx<tm1Clc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Crx<tm1Clc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Crx<tm1Clc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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