Mice homozygous for this knock-out mutation exhibit liver defects.
M. Bishr Omary, Stanford University & Palo Alto VA Medic
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Krt18 | keratin 18 |
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Hepatocytes are completely devoid of keratin filaments, but desmosomes are formed and maintained. At 18 months of age, mice consistently show a distinctive liver pathology with abnormal hepatocytes containing K8 (KRT8)-positive aggregates (Mallory bodies). No KRT18 protein is produced from the targeted gene, as determined by Western blot of liver tissue. This mutant mouse strain may be useful in studies pertaining to keratin 18 (Krt18) and its partner keratin 8 (Krt8) function in glandular tissues such as liver and pancreas.
A targeting vector was designed to replace exons 1 and part of 2 of the gene with an HPRT minigene driven by a mouse phosphoglycerate-kinase (PGK) promoter. Linearized vector was electroporated into 129P2/OlaHsd-derived HM-1 embryonic stem (ES) cells. Targeted clones were introduced to C57BL/6 or BALB/c blastocysts. The background of this strain is a mix of MF1, 129 and FVB. Upon arrival at The Jackson Laboratory, mutant mice were crossed to FVB/NJ inbred mice for at least one generation.
Allele Name | targeted mutation 1, Thomas M Magin |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | K18 null; K18- |
Gene Symbol and Name | Krt18, keratin 18 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd-Hprtb-m3 |
Chromosome | 15 |
Molecular Note | An HPRT minigene replaced most of exon 1, intron 1 and exon 2. |
Mutations Made By | Guozhong Tao, Stanford University |
When maintained as a live colony, heterozygotes or homozygotes may be used for breeding.
When using the K18 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007029 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Krt18<tm1Tmm> |
Frozen Mouse Embryo | STOCK Krt18<tm1Tmm>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Krt18<tm1Tmm>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Krt18<tm1Tmm>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Krt18<tm1Tmm>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.