Homozygous knock-out mice display impaired IL6 production in macrophages treated with an outer-surface lipoprotein.
Dr. Richard A. Flavell, Yale University School of Medicine
Homozygotes are viable and fertile. IL6 production is significantly impaired in homozygous macrophages treated with Borrelia burgdorferi's OspA (outer-surface lipoprotein). No expression products from the gene are found in either lipopolysacharide (LPS)-treated or untreated bone marrow-derived macrophages, as assayed by RT-PCR. This strain may be useful in vaccine development.
A targeting vector incorporating a loxP-flanked neomycin cassette in reverse orientation (derived from plasmid pMC1neo poly A, Stratagene) was used to replace the coding exon. The construct was introduced to 129S1/Sv-derived W9.5 embryonic stem (ES) cells. This resultant line has been backcrossed to C57BL/6 by the donating laboratory for ten generations.
|Allele Name||targeted mutation 1, Richard A Flavell|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Tlr1, toll-like receptor 1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||The entire gene was replaced by a neomycin resistance cassette. Rt-PCR confirmed the absence of transcription.|
|Mutations Made By|| |
Dr. Richard Flavell, Yale University School of Medicine
When maintained as a live colony, this strain may be bred using homozygous crosses.
When using the TLR1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #007020 in your Materials and Methods section.