Mice homozygous for this knock-out mutation display defective T cell activation and proliferation, abnormal immunoglobulin levels, and increased susceptibility to experimental autoimmune encephalomyelitis
Dr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Icos | inducible T cell co-stimulator |
Homozygotes are viable and fertile. T cell activation and proliferation are defective and the T cells fail to produce interleukin 4 (IL4) when differentiated in vitro or when primed in vivo. These mice show a greatly enhanced susceptibility to experimental autoimmune encephalomyelitis (EAE). Activated lymph node cells completely lack expression of the gene as determined by RT-PCR and flow cytometry. This strain may be useful in studies of inflammatory autoimmune diseases.
A targeting vector was designed to replace three exons encoding all of the amino acid sequences except the leader peptide with a neomycin gene driven by a PGK promoter. The targeting vector was transfected into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. This line has been backcrossed to C57BL/6 nine times by the donating laboratory.
Allele Name | targeted mutation 1, Richard A Flavell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ICOS- |
Gene Symbol and Name | Icos, inducible T cell co-stimulator |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 1 |
Molecular Note | The three exons for all of the amino acid coding sequence except the leader peptide were replaced with a PGK-neomycin resistance cassette. Absence of protein product was demonstrated immunologically. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintained as a live colony, heterozygotes or homozygotes may be used for breeding.
When using the ICOS KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007019 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Icos<tm1Flv> |
Frozen Mouse Embryo | B6.129S1-Icos<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Icos<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Icos<tm1Flv>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Icos<tm1Flv>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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