Mice homozygous for this knock-out mutation display defective T cell activation and proliferation, abnormal immunoglobulin levels, and increased susceptibility to experimental autoimmune encephalomyelitis
Dr. Richard A. Flavell, Yale University School of Medicine
Homozygotes are viable and fertile. T cell activation and proliferation are defective and the T cells fail to produce interleukin 4 (IL4) when differentiated in vitro or when primed in vivo. These mice show a greatly enhanced susceptibility to experimental autoimmune encephalomyelitis (EAE). Activated lymph node cells completely lack expression of the gene as determined by RT-PCR and flow cytometry. This strain may be useful in studies of inflammatory autoimmune diseases.
A targeting vector was designed to replace three exons encoding all of the amino acid sequences except the leader peptide with a neomycin gene driven by a PGK promoter. The targeting vector was transfected into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. This line has been backcrossed to C57BL/6 nine times by the donating laboratory.
|Allele Name||targeted mutation 1, Richard A Flavell|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Icos, inducible T cell co-stimulator|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||The three exons for all of the amino acid coding sequence except the leader peptide were replaced with a PGK-neomycin resistance cassette. Absence of protein product was demonstrated immunologically.|
|Mutations Made By|| |
Dr. Richard Flavell, Yale University School of Medicine
When maintained as a live colony, heterozygotes or homozygotes may be used for breeding.
When using the ICOS KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007019 in your Materials and Methods section.