B6.129S1-Icos^tm1Flv/J

Stock number: 007019
Product name: ICOS KO
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for this knock-out mutation display defective T cell activation and proliferation, abnormal immunoglobulin levels, and increased susceptibility to experimental autoimmune encephalomyelitis

Detailed description

Homozygotes are viable and fertile. T cell activation and proliferation are defective and the T cells fail to produce interleukin 4 (IL4) when differentiated in vitro or when primed in vivo. These mice show a greatly enhanced susceptibility to experimental autoimmune encephalomyelitis (EAE). Activated lymph node cells completely lack expression of the gene as determined by RT-PCR and flow cytometry. This strain may be useful in studies of inflammatory autoimmune diseases.

Development

A targeting vector was designed to replace three exons encoding all of the amino acid sequences except the leader peptide with a neomycin gene driven by a PGK promoter. The targeting vector was transfected into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. This line has been backcrossed to C57BL/6 nine times by the donating laboratory.

Allele

Symbol: Icos^tm1Flv
Name: targeted mutation 1, Richard A Flavell
MGI accession ID: MGI:2683635
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Icos
Marker name: inducible T cell co-stimulator
Chromosome: 1
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: The three exons for all of the amino acid coding sequence except the leader peptide were replaced with a PGK-neomycin resistance cassette. Absence of protein product was demonstrated immunologically.