STOCK Notch1^tm2Rko/GridJ

Stock number: 006951
Product name: Notch1^flox
Research areas: Cell Biology Research, Developmental Biology Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 1 of the Notch1 gene. This strain may be useful for generating tissue-specific conditional mutations for studying the development of a wide range of tissues.

Detailed description

Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.

When crossed to a strain expressing a differential Cre mediated reporter protein labeling: Notch1 signaling in actively cycling stem/progenitor cells (see Stock No. 006953), this mutant strain may be useful in studies of loss of Notch1 heterozygosity.

When bred to mice carrying Tg(Wnt1-cre)11Rth (Stock No. 009107), Cre recombinase expression in the midbrain and developing neural tube results in postnatal lethality.

Development

A targeting construct was designed to place a loxP-flanked PGK-neo cassette upstream of exon 1 of the targeted gene, as well as a single loxP site in intron 1. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase vector to remove the selection cassette. The resulting ES cells harboring the loxP-flanked exon 1 were injected into blastocysts to generate chimeric mice. Mutant mice were then sent to Dr. Thomas Gridley (The Jackson Laboratory) in 2003 on an unspecified mixed genetic background. These mice were supplied to The Jackson Laboratory Repository in 2007.

Allele

Symbol: Notch1^tm2Rko
Name: targeted mutation 2, Raphael Kopan
MGI accession ID: MGI:3044585
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Notch1
Marker name: notch 1
Chromosome: 2
Strain of origin: 129X1/SvJ
Molecular note: LoxP were inserted flanking the first coding exon of the gene. An adjacent loxP flanked neomycin cassette was removed by Cre-mediated recombination in ES cells prior to production of chimeric animals.