Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. <a href="https://www.jax.org/strain/003771">003771</a>), this mutant strain may be useful in studies of apoptosis in neural development.

When crossed to a strain expressing a differential Cre mediated reporter protein labeling: Notch1 signaling in actively cycling stem/progenitor cells (see Stock No. <a href="https://www.jax.org/strain/006953">006953</a>), this mutant strain may be useful in studies of loss of Notch1 heterozygosity.

When bred to mice carrying Tg(Wnt1-cre)11Rth (Stock No. <a href="https://www.jax.org/strain/009107">009107</a>), Cre recombinase expression in the midbrain and developing neural tube results in postnatal lethality.

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These floxed mutant mice possess loxP sites flanking exon 1 of the Notch1 gene. This strain may be useful for generating tissue-specific conditional mutations for studying the development of a wide range of tissues.

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