At 5 to 6 weeks of age, mice that are homozygous for this targeted mutation have a reduced number (approximately 68% of wildtype levels) of naive T cells in the spleen. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function. This strain was transferred from the collection of the Consortium for Functional Glycomics.
Steve Rosen, University of San Francisco
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of total spleen RNA. Protein activity is not detected in spleen, lung peripheral lymph nodes, mesenteric lymph nodes or Peyer's patches. At 5 to 6 weeks of age, homozygotes have a reduced number (approximately 68% of wildtype levels) of naive T cells in the spleen. Chondroitin sulfate D is not detected in the brains of adult homozygotes or in the telencephalon and cartilage of homozygote embryos aged 12.5 and 15.5 embryonic days. Brain development is not impaired in mutant mice. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function.
This strain was transferred from the collection of the Consortium for Functional Glycomics.
A targeting vector containing neomycin resistance and diphtheria toxin fragment A genes was used to disrupt the portion of exon 3 that encodes the catalytic domain. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to 129/SvJ mice, and then backcrossed to the same for 4 generations before being made homozygous. The mice were then backcrossed to C57BL/6 for 12 generations before arriving at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Takashi Muramatsu|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Chst3, carbohydrate sulfotransferase 3|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance cassette replaced sequences in exon III encoding part of the catalytic domain. Northern blot analysis of spleen did not detect mRNA in homozygous mutant mice. Protein activity was not detected in spleen, lung, and lymph nodes of homozygous mutant mice.|
|Mutations Made By|| |
Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Chst3tm1Tmu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006945 in your Materials and Methods section.