Overview

CD22 deficient mutant mice exhibit a reduction in recirculating mature B cells in the bone marrow and fewer transitional B cells in the spleen. Splenic and peripheral B cells express lower levels of membrane bound IgM than wildtype. B cell activation is abnormal in mutant mice, with enhanced Ca²⁺ mobilization after stimulation. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, B cell development and T cell independent immune response.

Donating Investigator

Lars Nitschke, University of Erlangen

Genetic overview

Genetic Background	Generation

Cd22^tm1Lam

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Cd22	CD22 antigen

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Cell Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No expression of the targeted gene's protein product is detected on the cell surface, as determined by flow cytometry analysis of splenocytes from homozygotes. Mutants exhibit a reduction in recirculating mature B cells in the bone marrow and fewer transitional B cells in the spleen. Splenic and peripheral B cells express lower levels of membrane bound IgM than wildtype. B cell activation is abnormal in mutant mice, with enhanced Ca²⁺ mobilization after stimulation. Lipopolysaccaharide (LPS) challenge results in an increased proliferative response. CD22 deficient B cells have a shorter than average lifespan when compared to wildtype B cells. T-cell independent immune responses are impaired. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, B cell development and T cell independent immune response.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon11, which encodes the transmembrane domain. The construct was electroporated into C57BL/6 derived BL/6-III embryonic stem (ES) cells.

Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reported that the mice were backcrossed to the C57BL/6 (see SNP note below) background for 10 generations before arriving at The Jackson Laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Nitschke L; Carsetti R; Ocker B; Kohler G; Lamers MC. 1997. CD22 is a negative regulator of B-cell receptor signalling. Curr Biol 7(2):133-43PubMed: 9016707 MGI: J:38644

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Genetics

Cd22^tm1Lam

Allele Symbol: Cd22^tm1Lam

Allele Name	targeted mutation 1, Marinus C Lamers
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	CD22-
Gene Symbol and Name	Cd22, CD22 antigen
Gene Synonym(s)
Strain of Origin	C57BL/6J
Chromosome	7
Molecular Note	Exon 11, encoding the transmembrane domain, was disrupted by the insertion of a neomycin cassette. Flow cytometric analysis showed an absence of surface expression on splenocytes isolated from homozygous mutant mice.
Mutations Made By	Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B cell deficiency
Cell Biology Research
- Protein Processing

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cd22^tm1Lam/Cd22^tm1Lam
involves: 129 * C57BL/6

hematopoietic system phenotype

abnormal B cell activation
- enhanced Ca²⁺ mobilization upon activation of B cells

decreased mature B cell number
- reduction of recirculating mature B cells in the bone marrow

decreased marginal zone B cell number
- reduced by 20%

immune system phenotype

decreased mature B cell number
- reduction of recirculating mature B cells in the bone marrow

abnormal B cell activation
- enhanced Ca²⁺ mobilization upon activation of B cells

decreased marginal zone B cell number
- reduced by 20%

References

Nitschke L; Carsetti R; Ocker B; Kohler G; Lamers MC. 1997. CD22 is a negative regulator of B-cell receptor signalling. Curr Biol 7(2):133-43PubMed: 9016707 MGI: J:38644

Additional - Cd22^tm1Lam related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Cd22
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the C57BL/6-Cd22^tm1Lam /J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006940 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Heterozygous for Cd22<tm1Lam>

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Cd22tm1Lam

Allele Symbol: Cd22tm1Lam

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Cd22tm1Lam/Cd22tm1Laminvolves: 129 * C57BL/6

hematopoietic system phenotype

immune system phenotype

References

Additional - Cd22tm1Lam related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Cd22^tm1Lam

Allele Symbol: Cd22^tm1Lam

Genotype: Cd22^tm1Lam/Cd22^tm1Lam
involves: 129 * C57BL/6

Additional - Cd22^tm1Lam related